Apolipoprotein A-I/ApoA1 Antibody Pair [HRP] Summary
Description |
Solid Phase sandwich ELISA for the quantitative determination of Human Apolipoprotein A-I/ApoA1. |
Specificity |
This solid phase sandwich ELISA utilizes an antibody specific for Human Apolipoprotein A-I/ApoA1. |
Standard Curve Range |
250-8000 pg/ml (example only; lot dependent) |
Sensitivity |
250 pg/ml (example only; lot dependent) |
Assay Type |
Sandwich ELISA |
Sample Volume |
100 ul |
Gene |
APOA1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Rabbit Polyclonal Detection Antibody (HRP-conjugated)
- Standard
|
Alternate Names for Apolipoprotein A-I/ApoA1 Antibody Pair [HRP]
Background
Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are
guaranteed for 6 months from date of receipt.
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