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Apolipoprotein A-I/ApoA1 Antibody (JF0548)

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Western Blot: Apolipoprotein A-I/ApoA1 Antibody (JF0548) [NBP2-67161] - Analysis of Apolipoprotein A1 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour ...read more
Immunocytochemistry/ Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody (JF0548) [NBP2-67161] - Staining Apolipoprotein A1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody (JF0548) [NBP2-67161] - Analysis of paraffin-embedded mouse liver tissue using anti-Apolipoprotein A1 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody (JF0548) [NBP2-67161] - Staining Apolipoprotein A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunocytochemistry/ Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody (JF0548) [NBP2-67161] - Staining Apolipoprotein A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody (JF0548) [NBP2-67161] - Analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein A1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
JF0548
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Apolipoprotein A-I/ApoA1 Antibody (JF0548) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within human Apolipoprotein A-I/ApoA1 aa 1-50. (SwissProt: P02647 Human; SwissProt: Q00623 Mouse)
Localization
Secreted.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
APOA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody (JF0548)

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol APOA1