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APAF-1 Antibody

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Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP2-15420] - Paraffin-embedded mouse duodenum. APAF1 antibody [C3], C-term diluted at 1:400.
Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP2-15420] - Paraffin-embedded mouse duodenum. APAF1 antibody [C3], C-term diluted at 1:400.
Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP2-15420] - Paraffin-embedded breast ca, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP2-15420] - Paraffin-embedded rat liver. APAF1 antibody [C3], C-term diluted at 1:400.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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APAF-1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human APAF-1. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APAF1
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for APAF-1 Antibody

  • APAF1
  • APAF-1
  • APAF-1apoptotic protease activating factor 1
  • apoptotic peptidase activating factor 1
  • apoptotic peptidase activating factor
  • apoptotic protease activating factor
  • apoptotic protease-activating factor 1
  • CED4
  • DKFZp781B1145
  • KIAA0413

Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APAF1
Uniprot