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Annexin V Antibody [Unconjugated]

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Western blot shows lysates of human lung tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Annexin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Annexin V at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human lung tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Annexin V Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of Human lung, mouse lung, rat lung, loaded at 0.2 mg/mL. A specific band was detected for Annexin V at approximately 38 kDa (as indicated) using 12.5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF399
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF399-SP
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Annexin V Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Annexin V
Ala2-Asp320
Accession # P08758
Specificity
Detects human, mouse and rat Annexin V in Western blots. Detects human Annexin V in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANXA5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 12.5 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF399 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin V Antibody [Unconjugated]

  • Anchorin CII
  • annexin A5
  • Annexin V
  • annexin-5
  • ANX5
  • ANXA5
  • ANXV
  • Calphobindin I
  • Endonexin II
  • ENX2CBP-I
  • Lipocortin V
  • PAP-I
  • Placental anticoagulant protein 4
  • Placental anticoagulant protein I
  • PP4
  • Thromboplastin inhibitor
  • VAC-alpha
  • Vascular anticoagulant-alpha

Background

Annexin V is a member of a calcium and phospholipid binding family of proteins with vascular anticoagulant activity. It is also known as PP4, PAP I, CPB-I, CaBP33, VACa, anchorin CII, lipocortin-V, endonexin II, and thromboplastin inhibitor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Annexin V Antibody (AF399)(9)

We have publications tested in 2 confirmed species: Mouse, Porcine.

We have publications tested in 3 applications: Flow Cytometry, Microarray, Western Blot.


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Flow Cytometry
(1)
Microarray
(1)
Western Blot
(2)
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Mouse
(4)
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(1)
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Showing Publications 1 - 9 of 9.
Publications using AF399 Applications Species
K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers Theriogenology, 2020-04-04;149(0):104-116. 2020-04-04 [PMID: 32259747] (Microarray, Mouse) Microarray Mouse
Alexandra Brahmer, Elmo Neuberger, Leona Esch-Heisser, Nils Haller, Malene Moeller Jorgensen, Rikke Baek et al. Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation Journal of Extracellular Vesicles 2019-12-01 [PMID: 31191831]
Anne Louise Schacht Revenfeld, Rikke Bæk, Malene Møller Jørgensen, Kim Varming, Allan Stensballe Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation International Journal of Molecular Sciences 2017-07-24 [PMID: 28737722]
Lotte Hatting Pugholm, Rikke Bæk, Evo Kristina Lindersson Søndergaard, Anne Louise Schacht Revenfeld, Malene Møller Jørgensen, Kim Varming Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles Journal of Immunology Research 2016-04-18 [PMID: 27195303]
Joseph El Khoury Megf10 Is a Receptor for C1Q That Mediates Clearance of Apoptotic Cells by Astrocytes J. Neurosci., 2016-05-11;36(19):5185-92. 2016-05-11 [PMID: 27170117] (Mouse) Mouse
Linke B, Abeler-Dorner L, Jahndel V, Kurz A, Mahr A, Pfrang S, Linke L, Krammer P, Weyd H The tolerogenic function of annexins on apoptotic cells is mediated by the annexin core domain. J Immunol, 2015-04-27;194(11):5233-42. 2015-04-27 [PMID: 25917090] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Jorgensen MM, Baek R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles 1/1/2015 [PMID: 25862471]
Jubeck B, Muth E, Gohr CM, Rosenthal AK Type II collagen levels correlate with mineralization by articular cartilage vesicles. Arthritis Rheum., 2009-09-01;60(9):2741-6. 2009-09-01 [PMID: 19714645] (Western Blot, Porcine) Western Blot Porcine
McDonald TG, Scott SA, Kane KM, Kawaja MD Proteomic assessment of sympathetic ganglia from adult mice that possess null mutations of ExonIII or ExonIV in the p75 neurotrophin receptor gene. Brain Res., 2008-11-18;1253(0):1-14. 2008-11-18 [PMID: 19046947] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol ANXA5
Uniprot