![Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12] - Analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody (M12), clone 4F1. Lane 1: ANXA3 transfected lysatE (37.145 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Annexin-A3-Antibody-4F1-Western-Blot-H00000306-M12-img0004.jpg "Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12] - Analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody (M12), clone 4F1. Lane 1: ANXA3 transfected lysatE (37.145 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, KD |
| Clone | 4F1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | ANXA3 (AAH00871, 1 a.a. ~ 323 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGDD |
| Specificity | ANXA3 (4F1) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ANXA3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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| Publications using H00000306-M12 | Applications | Species |
|---|---|---|
| Hojun K, Sungwook P, In J et al. Noninvasive Precision Screening of Prostate Cancer by Urinary Multimarker Sensor and Artificial Intelligence Analysis. ACS Nano. 2020-12-09 [PMID: 33296173] | ||
| Watanabe T, Ito Y, Sato A et al. Annexin A3 as a Negative Regulator of Adipocyte Differentiation. J Biochem. 2012-08-09 [PMID: 22888117] | ||
| Cao DL, Ye DW, Zhang HL et al. A multiplex model of combining gene-based, protein-based, and metabolite-based with positive and negative markers in urine for the early diagnosis of prostate cancer. Prostate 2010-10-18 [PMID: 20957673] | ||
| Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01 |
Secondary Antibodies |
Isotype Controls |
Research Areas for Annexin A3 Antibody (H00000306-M12)Find related products by research area.
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![Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12] - Western blot analysis of ANXA3 over-expressed 293 cell line. Lane 2 cotransfected with ANXA3 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000306-M12. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/Annexin-A3-Antibody-4F1-Western-Blot-H00000306-M12-img0002.jpg "Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12] - Western blot analysis of ANXA3 over-expressed 293 cell line. Lane 2 cotransfected with ANXA3 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000306-M12. GAPDH (36.1 kDa) used as loading control.")
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