![Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Localization of annexin A2 at the focal adhesions: KS1767 cells co-stained for paxillin (FITC-conjugated secondary antibody, green) and annexin A2 (Alexa 647-conjugated secondary antibody, red). An enlarged inset (white dotted line) is included for prompt visualization. Nuclei are visualized with DAPI (blue); scale bar, 10um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-03470-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Annexin-A2-Antibody-1G7-Immunocytochemistry-Immunofluorescence-H00000302-M02-img0007.jpg "Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Localization of annexin A2 at the focal adhesions: KS1767 cells co-stained for paxillin (FITC-conjugated secondary antibody, green) and annexin A2 (Alexa 647-conjugated secondary antibody, red). An enlarged inset (white dotted line) is included for prompt visualization. Nuclei are visualized with DAPI (blue); scale bar, 10um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-03470-w), licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IP |
| Clone | 1G7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | ANXA2 (AAH66955, 19 a.a. ~ 357 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISVMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD |
| Specificity | ANXA2 - annexin A2 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ANXA2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Yongkang Yang |
WB | Human | 09/12/2021 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Co-localization analysis: KS1767 cells grown overnight onto circular coverslips were incubated for 2h at 37degrees C with biotinylated LGRFYAASG-pen, or unconjugated LGRFYAASG (10uM). Untreated cells were used as controls. IF staining of biotinylated peptides (avidin-FITC, green) and annexin A2 (Cy3-conjugated secondary antibody, red) was analyzed with Fiji ImageJ. Co-localized pixels are visualized in white (arrows), and Manders' overlap coefficients are reported in the graph (**P<0.01; ***P<0.001 versus LGRFYAASG-pen). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-03470-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Annexin-A2-Antibody-1G7-Immunocytochemistry-Immunofluorescence-H00000302-M02-img0008.jpg "Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Co-localization analysis: KS1767 cells grown overnight onto circular coverslips were incubated for 2h at 37degrees C with biotinylated LGRFYAASG-pen, or unconjugated LGRFYAASG (10uM). Untreated cells were used as controls. IF staining of biotinylated peptides (avidin-FITC, green) and annexin A2 (Cy3-conjugated secondary antibody, red) was analyzed with Fiji ImageJ. Co-localized pixels are visualized in white (arrows), and Manders' overlap coefficients are reported in the graph (**P<0.01; ***P<0.001 versus LGRFYAASG-pen). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-03470-w), licensed under a CC-BY license.")
![Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - MDA-MB-231 subclones whole cell lysates were loaded with 30 ug/lane. 10% SDS-PAGE. ANXA2 Antibody (H00000302-M02) was used for primary antibody: 1:2000, 4C, overnight. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Annexin-A2-Antibody-1G7-Western-Blot-H00000302-M02-img0009.jpg "Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - MDA-MB-231 subclones whole cell lysates were loaded with 30 ug/lane. 10% SDS-PAGE. ANXA2 Antibody (H00000302-M02) was used for primary antibody: 1:2000, 4C, overnight. WB image submitted by a verified customer review.")
![Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 monoclonal antibody (M02), clone 1G7 Analysis of ANXA2 expression in HepG2.](http://images.novusbio.com/fullsize/Annexin-A2-Antibody-1G7-Western-Blot-H00000302-M02-img0005.jpg "Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 monoclonal antibody (M02), clone 1G7 Analysis of ANXA2 expression in HepG2.")
![Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M02), clone 1G7.Lane 1: ANXA2 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Annexin-A2-Antibody-1G7-Western-Blot-H00000302-M02-img0006.jpg "Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M02), clone 1G7.Lane 1: ANXA2 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of monoclonal antibody to ANXA2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Annexin-A2-Antibody-1G7-Immunocytochemistry-Immunofluorescence-H00000302-M02-img0004.jpg "Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of monoclonal antibody to ANXA2 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 could regulate the activation of NF-kappa B through combined with the subunit p50. a, b. The change in localization of endogenous p50 was analyzed by IF-IC following treatment with or without TNF-alpha (100 ng/ml) for 1 h. Nuclei were stained with DAPI. The nuclear localization of endogenous p50 in each group were quantitated as a graph. The plotted error bars represent mean ± SEM. The nuclear localization of p50 was quantified according to data from 5 random figures in each group. c. Interaction between ANXA2 & p50 in SK-N-BE(2) cells was validated through Co-IP & analyzed by western blotting with the indicated antibodies **: P < 0.01. All results are from at least three independent experiments Image collected & cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-017-0581-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000302-m02_mouse-monoclonal-annexin-a2-antibody-1g7-310202415395957.jpg "Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 could regulate the activation of NF-kappa B through combined with the subunit p50. a, b. The change in localization of endogenous p50 was analyzed by IF-IC following treatment with or without TNF-alpha (100 ng/ml) for 1 h. Nuclei were stained with DAPI. The nuclear localization of endogenous p50 in each group were quantitated as a graph. The plotted error bars represent mean ± SEM. The nuclear localization of p50 was quantified according to data from 5 random figures in each group. c. Interaction between ANXA2 & p50 in SK-N-BE(2) cells was validated through Co-IP & analyzed by western blotting with the indicated antibodies **: P < 0.01. All results are from at least three independent experiments Image collected & cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-017-0581-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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