![Western Blot: Angiogenin Antibody [NBP2-41185] - Angiogenin and EPCs modulation after human stroke and rehabilitation. (A) Infarct tissue and contralateral brain homogenates (less than or equal 4 days) were analyzed by western blot for angiogenin (n = 3). The densitometry results (arbitrary units) were corrected by the actin load and bar graph showing the angiogenin fold-change of the ipsilateral vs. contralateral signal. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fneur.2018.00508/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Angiogenin-Antibody-Western-Blot-NBP2-41185-img0003.jpg "Western Blot: Angiogenin Antibody [NBP2-41185] - Angiogenin and EPCs modulation after human stroke and rehabilitation. (A) Infarct tissue and contralateral brain homogenates (less than or equal 4 days) were analyzed by western blot for angiogenin (n = 3). The densitometry results (arbitrary units) were corrected by the actin load and bar graph showing the angiogenin fold-change of the ipsilateral vs. contralateral signal. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fneur.2018.00508/full) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 15 amino acid synthetic peptide near the center of human ANG. The immunogen is located within amino acids 70 - 120 of ANG. Amino Acid Squence: NKNGNPHRENLRISKS |
| Specificity | Two alternatively spliced transcript variants have been observed. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANG |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in IHC reported in scientific literature (PMID: 30008694). |
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| Theoretical MW | 16 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
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| Publication using NBP2-41185 | Applications | Species |
|---|---|---|
| Gabriel-Salazar M, Morancho A, Rodriguez S et al. Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia. Front Neurol. 2018-06-29 [PMID: 30008694] (IF/IHC, WB, Human) | IF/IHC, WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Angiogenin Antibody (NBP2-41185)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ANG |
![Immunocytochemistry/Immunofluorescence: Angiogenin Antibody [NBP2-41185] - Human liver tissue with ANG antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Angiogenin-Antibody-Immunofluorescence-NBP2-41185-img0002.jpg "Immunocytochemistry/Immunofluorescence: Angiogenin Antibody [NBP2-41185] - Human liver tissue with ANG antibody at 20 ug/mL.")
![Western Blot: Angiogenin Antibody [NBP2-41185] - Analysis of ANG in rat liver tissue lysate with Ang antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Angiogenin-Antibody-Western-Blot-NBP2-41185-img0001.jpg "Western Blot: Angiogenin Antibody [NBP2-41185] - Analysis of ANG in rat liver tissue lysate with Ang antibody at (A) 1 and (B) 2 ug/mL.")
![Western Blot: Angiogenin Antibody - BSA Free [NBP2-41185] - Angiogenin & EPCs modulation after mouse cerebral ischemia & rehabilitation. (A) Infarct & contralateral mouse brain cortical homogenates were analyzed by western blot to quantify angiogenin protein (n = 3). The densitometry results (arbitrary units) were corrected by the actin load & are represented as fold-change of the ipsilateral vs. contralateral signal. (B) Bar graphs representing the angiogenin RNA expression in the ischemic & contralateral cortex in the short-term (n = 6–9) & long-term rehabilitation groups (n = 5), **p < 0.01, *p < 0.05 as indicated by horizontal lines. Non-RHB ipsilateral short-term vs. long-term; &p < 0.05. Pasta Matrix & Treadmill contralateral short-term vs. long-term; †p < 0.05. (C) Bar graphs showing the percentage of the Angiogenin+ area in the pasta matrix long-term rehabilitation group (n = 6) together with images of representative brains with inserts showing co-localization of angiogenin in neurons: scale bar represents 100 μm. *p < 0.05. (D) Graph showing the plasma angiogenin temporal profile of No-RHB, Pasta matrix, & Treadmill groups (n = 3–4/group); *p < 0.05. (E) Box plots representing the cell density of EPCs from the three different RHB groups (n = 3–5 short-term; n = 6–7 long-term) & representative images of the primary cultures; scale bar represents 250 μm. Data are represented as the mean ± SEM or as box plots indicating the median (IQR). IP, ipsilateral; CL, contralateral; ST-R, short-term rehabilitation; LT-R, long-term rehabilitation; No-RHB, No-Rehabilitation; EPC, endothelial progenitor cells. Image collected & cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fneur.2018.00508/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-41185_rabbit-polyclonal-angiogenin-antibody-31020241612238.jpg "Western Blot: Angiogenin Antibody - BSA Free [NBP2-41185] - Angiogenin & EPCs modulation after mouse cerebral ischemia & rehabilitation. (A) Infarct & contralateral mouse brain cortical homogenates were analyzed by western blot to quantify angiogenin protein (n = 3). The densitometry results (arbitrary units) were corrected by the actin load & are represented as fold-change of the ipsilateral vs. contralateral signal. (B) Bar graphs representing the angiogenin RNA expression in the ischemic & contralateral cortex in the short-term (n = 6–9) & long-term rehabilitation groups (n = 5), **p < 0.01, *p < 0.05 as indicated by horizontal lines. Non-RHB ipsilateral short-term vs. long-term; &p < 0.05. Pasta Matrix & Treadmill contralateral short-term vs. long-term; †p < 0.05. (C) Bar graphs showing the percentage of the Angiogenin+ area in the pasta matrix long-term rehabilitation group (n = 6) together with images of representative brains with inserts showing co-localization of angiogenin in neurons: scale bar represents 100 μm. *p < 0.05. (D) Graph showing the plasma angiogenin temporal profile of No-RHB, Pasta matrix, & Treadmill groups (n = 3–4/group); *p < 0.05. (E) Box plots representing the cell density of EPCs from the three different RHB groups (n = 3–5 short-term; n = 6–7 long-term) & representative images of the primary cultures; scale bar represents 250 μm. Data are represented as the mean ± SEM or as box plots indicating the median (IQR). IP, ipsilateral; CL, contralateral; ST-R, short-term rehabilitation; LT-R, long-term rehabilitation; No-RHB, No-Rehabilitation; EPC, endothelial progenitor cells. Image collected & cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fneur.2018.00508/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: Angiogenin Antibody - BSA Free [NBP2-41185] - Immunofluorescence of Angiogenin in human liver tissue with Angiogenin antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41185_rabbit-polyclonal-angiogenin-antibody-2711202419333490.jpg "Immunocytochemistry/ Immunofluorescence: Angiogenin Antibody - BSA Free [NBP2-41185] - Immunofluorescence of Angiogenin in human liver tissue with Angiogenin antibody at 20 u/mL.")
![Immunocytochemistry/ Immunofluorescence: Angiogenin Antibody - BSA Free [NBP2-41185] - Immunofluorescence of Angiogenin in human liver tissue with Angiogenin antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41185_rabbit-polyclonal-angiogenin-antibody-2711202420171783.jpg "Immunocytochemistry/ Immunofluorescence: Angiogenin Antibody - BSA Free [NBP2-41185] - Immunofluorescence of Angiogenin in human liver tissue with Angiogenin antibody at 20 u/mL.")
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