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Aminopeptidase B/RNPEP Antibody

Images

 
Independent Antibodies: Western Blot: Aminopeptidase B/RNPEP Antibody [NBP2-54975] - Analysis using Anti-RNPEP antibody NBP2-54975 (A) shows similar pattern to independent antibody NBP1-85897 (B).
Immunocytochemistry/ Immunofluorescence: Aminopeptidase B/RNPEP Antibody [NBP2-54975] - Staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
Immunohistochemistry-Paraffin: Aminopeptidase B/RNPEP Antibody [NBP2-54975] - Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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Aminopeptidase B/RNPEP Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MKPAEELAQLWAAEELDMKAIEAVAISPWKTYQLVYFLDKILQKSPLPPGNVKKLGDTYPSISNARNAELRLRWGQI
Predicted Species
Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNPEP
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Aminopeptidase B/RNPEP Recombinant Protein Antigen (NBP2-54975PEP)

Reactivity Notes

Mouse 87%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Aminopeptidase B/RNPEP Antibody

  • Aminopeptidase B
  • APB
  • AP-B
  • Arginine aminopeptidase
  • arginyl aminopeptidase (aminopeptidase B)
  • Arginyl Aminopeptidase
  • DKFZp547H084
  • EC 3.4.11
  • EC 3.4.11.6
  • RNPEP

Background

Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RNPEP