Reactivity | HuSpecies Glossary |
Applications | Flow |
Clone | 767727 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Alexa Fluor 750 |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human Allergin-1 Met1-Lys227 Accession # Q7Z6M3 |
Specificity | Detects human Allergin-1 in direct ELISAs. |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Mouse |
Gene | MILR1 |
Purity Statement | Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative | 0.09% Sodium Azide |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
MCA32 (Mast Cell Antigen; also Allergin‑1 and c17orf60) is a 70 kDa member of the Ig Superfamily. It is expressed on mast cells, basophils, neutrophils and macrophages, and is reported to negatively regulate allergic mediator release from mast cells. In particular, MCA32 interacts with Fc epsilon RI. Upon IgE mediated Fc epsilon RI aggregation and activation, Allergin‑1 recruits Tyr phosphatases to a Fc epsilon RI tetrameric complex, dampening IgE receptor downstream signaling. Mature human Allergin‑1 is a monomeric type I transmembrane glycoprotein that is 324 amino acids (aa) in length. It contains two C2-type Ig domains (aa 35-118 and 123-217) in the extracellular region (aa 20-227), plus a 95 aa cytoplasmic domain that possesses two ITIM motifs. Two 40 kDa splice variants exist that show deletions of the Ig-like domains. One isoform termed Allergin‑1S1 is missing aa 128-217, while a second isoform called Allergin‑1S2 is missing aa 33-122. This second isoform represents the antigen used for immunization in this product (GenBank BAJ08253). Over aa 29-131, the Allergin‑1S2 isoform shares 100% sequence identity with human Allergin‑1L full length isoform and only 24% aa sequence identity with mouse Allergin‑1.
Secondary Antibodies |
Isotype Controls |
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