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Albumin Antibody [Unconjugated]

Images

 
Western blot shows lysates of human liver tissue and mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) ...read more
Serum Albumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) at 10 µg/mL for 3 ...read more
Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat ...read more
Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line undifferentiated (0) and hepatocyte differentiated for 14 and 20 days, loaded at 0.2 mg/mL. A specific band was detected for ...read more
Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Serum Albumin ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

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Catalog# & Formulation Size Price
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Albumin Antibody [Unconjugated] Summary

Immunogen
Mouse Serum Albumin
Specificity
Detects human and mouse Serum Albumin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALB
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 5-10 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3329 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Albumin Antibody [Unconjugated]

  • ALB
  • Albumin
  • cell growth inhibiting protein 42
  • DKFZp779N1935
  • growth-inhibiting protein 20
  • PRO0883
  • PRO0903
  • PRO1341
  • serum albumin

Background

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Albumin Antibody (AF3329)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 5 applications: Functional Assay, ICC, IHC, Immunohistochemistry, Western Blot.


Filter By Application
Functional Assay
(1)
ICC
(1)
IHC
(3)
Immunohistochemistry
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF3329 Applications Species
Hu, Z;Cano, I;Lei, F;Liu, J;Ramos, RB;Gordon, H;Paschalis, EI;Saint-Geniez, M;Ng, YSE;D'Amore, PA; Deletion of the endothelial glycocalyx component endomucin leads to impaired glomerular structure and function bioRxiv : the preprint server for biology 2024-07-19 [PMID: 39071302] (Western Blot, Mouse) Western Blot Mouse
Anagnostopoulos, G;Saavedra, E;Lambertucci, F;Motiño, O;Dimitrov, J;Roiz-Valle, D;Quesada, V;Alvarez-Valadez, K;Chen, H;Sauvat, A;Rong, Y;Nogueira-Recalde, U;Li, S;Montégut, L;Djavaheri-Mergny, M;Castedo, M;Lopez-Otin, C;Maiuri, MC;Martins, I;Kroemer, G; Inhibition of acyl-CoA binding protein (ACBP) by means of a GABAAR?2-derived peptide Cell death & disease 2024-04-06 [PMID: 38582872] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
F Alonso, L Li, I Fremaux, DP Reinhardt, E Génot Fibrillin-1 Regulates Arteriole Integrity in the Retina Biomolecules, 2022-09-20;12(10):. 2022-09-20 [PMID: 36291539] (IHC, Mouse) IHC Mouse
SJ Park, Y Kim, C Li, J Suh, J Sivapackia, TM Goncalves, G Jarad, G Zhao, F Urano, V Sharma, YM Chen Blocking CHOP-dependent TXNIP shuttling to mitochondria attenuates albuminuria and mitigates kidney injury in nephrotic syndrome Proceedings of the National Academy of Sciences of the United States of America, 2022-08-22;119(35):e2116505119. 2022-08-22 [PMID: 35994650] (IHC, Mouse, Transgenic Mouse) IHC Mouse, Transgenic Mouse
Diego Gomes, Shari Wang, Leela Goodspeed, Katherine E. Turk, Tomasz Wietecha, Yongjun Liu et al. Comparison between genetic and pharmaceutical disruption of Ldlr expression for the development of atherosclerosis Journal of Lipid Research 3/1/2022 [PMID: 35101425]
I Hwang, EJ Lee, H Park, D Moon, HS Kim Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis Cell & bioscience, 2021-01-06;11(1):3. 2021-01-06 [PMID: 33407858] (ICC, IHC, Human, Mouse, Transgenic Mouse) ICC, IHC Human, Mouse, Transgenic Mouse
Mengyun Xiao, Bernhard N. Bohnert, Hande Aypek, Oliver Kretz, Florian Grahammer, Ute Aukschun et al. Plasminogen deficiency does not prevent sodium retention in a genetic mouse model of experimental nephrotic syndrome Acta Physiol (Oxf) 1/1/2021 [PMID: 32455507]
Opposing impacts on healthspan and longevity by limiting dietary selenium in telomere dysfunctional mice Aging Cell, 2016-09-21;0(0):. 2016-09-21 [PMID: 27653523] (Functional Assay, Mouse) Functional Assay Mouse

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Secondary Antibodies

 

Isotype Controls

Additional Albumin Products

Blogs on Albumin.

Albumin - a family of globular protein
Albumin is a soluble and globular monomeric protein encoded by chromosome 4 that comprises about half of the protein found in blood serum. It functions as a carrier protein for steroids, fatty acids, and thyroid hormones as well as stabilizing extrace...  Read full blog post.

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Bioinformatics

Gene Symbol ALB