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AKR1C2 Overexpression Lysate

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Western Blot: AKR1C2 Overexpression Lysate (Adult Normal) [NBP2-04548] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1C2.
Western Blot: AKR1C2 Overexpression Lysate (Adult Normal) [NBP2-04548] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1C2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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AKR1C2 Overexpression Lysate Summary

Description

AKR1C2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC209081

Accession#: NM_205845

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
AKR1C2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
36.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for AKR1C2 Overexpression Lysate

  • aldo-keto reductase family 1 member C2
  • aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acidbinding protein; 3-alpha hydroxysteroid dehydrogenase, type III)
  • BABP
  • Chlordecone reductase homolog HAKRD
  • DD
  • DD-2
  • DD2DD/BABP
  • DDH2FLJ53800
  • Dihydrodiol dehydrogenase 2
  • Dihydrodiol dehydrogenase/bile acid-binding protein
  • EC 1.1.1,3-alpha-HSD3
  • EC 1.1.1.213
  • EC 1.3.1.20
  • HAKRDAKR1C-pseudo
  • HBAB
  • MCDR2
  • pseudo-chlordecone reductase
  • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
  • type II dihydrodiol dehydrogenase
  • Type III 3-alpha-hydroxysteroid dehydrogenase

Background

AKR1C2 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AKR1C2