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Aiolos/IKZF3 Antibody (S01-0C5)

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Western Blot: Aiolos/IKZF3 Antibody (S01-0C5) [NBP3-20069] - Western blot detection of Aiolos/IKZF3 in HL-60, U251 cell lysates using NBP3-20069 (1:1000 diluted). Predicted band size: 58kDa. Observed band size: 58kDa.
Western Blot: Aiolos/IKZF3 Antibody (S01-0C5) [NBP3-20069] - Western blot detection of Aiolos/IKZF3 in HL-60, U251 cell lysates using NBP3-20069 (1:1000 diluted). Predicted band size: 58kDa. Observed band size: 58kDa.
Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody (S01-0C5) [NBP3-20069] - Immunohistochemistry of Aiolos/IKZF3 in paraffin-embedded Human tonsil using NBP3-20069 at dilution 1/50

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
S01-0C5
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

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Aiolos/IKZF3 Antibody (S01-0C5) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human Aiolos/IKZF3 (Uniprot # Q9UKT9)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IKZF3
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for Aiolos/IKZF3 Antibody (S01-0C5)

  • AIO
  • Aiolos
  • IKAROS family zinc finger 3 (Aiolos)
  • Ikaros family zinc finger protein 3
  • IKZF3
  • zinc finger protein Aiolos
  • ZNFN1A3
  • ZNFN1A3subfamily 1A, 3 (Aiolos)

Background

IKZF3 encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IKZF3