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Aiolos/IKZF3 Antibody (4U7F9)

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Western Blot: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Western blot analysis of extracts of various cell lines, using Aiolos/IKZF3 antibody (NBP3-16668) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit ...read more
Immunohistochemistry: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Immunofluorescence analysis of mouse spleen using Aiolos/IKZF3 Rabbit mAb (NBP3-16668) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Western blot analysis of extracts of Raji cells, using Aiolos/IKZF3 antibody (NBP3-16668) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ...read more
Western Blot: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Western blot analysis of extracts of Rat lung, using Aiolos/IKZF3 antibody (NBP3-16668) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Aiolos/IKZF3 antibody (NBP3-16668) at dilution of 1:100 (40x ...read more
Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Immunohistochemistry of paraffin-embedded human tonsil using Aiolos/IKZF3 antibody (NBP3-16668) at dilution of 1:100 (40x lens).Perform high ...read more
Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Immunohistochemistry of paraffin-embedded mouse spleen using Aiolos/IKZF3 antibody (NBP3-16668) at dilution of 1:100 (40x lens).Perform high ...read more
Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Immunohistochemistry of paraffin-embedded rat lung using Aiolos/IKZF3 antibody (NBP3-16668) at dilution of 1:100 (40x lens).Perform high ...read more
Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Immunohistochemistry of paraffin-embedded rat spleen using Aiolos/IKZF3 antibody (NBP3-16668) at dilution of 1:100 (40x lens).Perform high ...read more
Immunohistochemistry: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Immunofluorescence analysis of rat spleen using Aiolos/IKZF3 Rabbit mAb (NBP3-16668) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
4U7F9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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Aiolos/IKZF3 Antibody (4U7F9) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-509 of human Aiolos/IKZF3 (Q9UKT9). IYQQNHMVLSRARNGMPLLKEVPRSYELLKPPPICPRDSVKVINKEGEVMDVYRCDHCRVLFLDYVMFTIHMGCHGFRDPFECNMCGYRSHDRYEFSSHIARGEHRALLK
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IKZF3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Aiolos/IKZF3 Antibody (4U7F9)

  • AIO
  • Aiolos
  • IKAROS family zinc finger 3 (Aiolos)
  • Ikaros family zinc finger protein 3
  • IKZF3
  • zinc finger protein Aiolos
  • ZNFN1A3
  • ZNFN1A3subfamily 1A, 3 (Aiolos)

Background

IKZF3 encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IKZF3