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AG-2/AGR2 Antibody

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Orthogonal Strategies: Western Blot: AG-2 Antibody [NBP1-89212] - Analysis in human cell lines A-549 and HEK293 using anti-AGR2 antibody. Corresponding AGR2 RNA-seq data are presented for the same cell lines. ...read more
Immunocytochemistry/ Immunofluorescence: AG-2 Antibody [NBP1-89212] - Staining of human cell line RT4 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Analysis in human stomach and skeletal muscle tissues. Corresponding AGR2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human stomach shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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AG-2/AGR2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RDTTVKPGAKKDTKDSRPKLPQTLSRGWGDQLIWTQTYEEALYKSKTSNKPLMIIHHLDECPHSQALKKVFAENKEIQKLAEQFVLLNLVYETTDKHLSPDGQYVPRIMFVDPSLTVRADITGR
Predicted Species
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
AG-2/AGR2 Recombinant Protein Antigen (NBP1-89212PEP)
Publications
Read Publications using NBP1-89212.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AG-2/AGR2 Antibody

  • AG2
  • AG-2
  • AG2member 17
  • AGR2
  • anterior gradient 2 homolog (Xenopus laevis)
  • anterior gradient homolog 2 (Xenopus laevis)
  • GOB-4
  • hAG-2
  • HPC8
  • PDIA17
  • secreted cement gland homolog

Background

Anterior gradient homolog 2 (AGR2) is a proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. AGR2 is expressed in a number of different tumor types and high levels of AGR2 correlate with downregulation of the p53 response. AGR2 is being investigated for its use as a potential diagnostic and therapeutic target in various cancers, and AGR2 antibodies are important tools for cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AG-2/AGR2 Antibody (NBP1-89212)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AG-2/AGR2 Antibody (NBP1-89212) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AGR2