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Recombinant Rat Adiponectin/Acrp30 FLAG Protein

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Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.
Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Bioactivity
Concentration
LYOPH

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Recombinant Rat Adiponectin/Acrp30 FLAG Protein Summary

Description
Rat adiponectin (aa 16-244) fused at the N-terminus to a FLAG(R)-tag.

Source: HEK 293 cells Uniprot ID: Q8K3R4

TMW: ~30kDa (SDS-PAGE); ~180kDa (Hexamer, by SEC)
Details of Functionality
Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells
Source
HEK293
Protein/Peptide Type
Recombinant Protein
Gene
ADIPOQ
Purity
>95% pure by SDS-PAGE
Endotoxin Note
<0.01EU/ug purified protein (LAL test).

Applications/Dilutions

Dilutions
  • Bioactivity
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 30mM Tris-Cl, pH 8.0 + 100mM NaCl
Preservative
No Preservative
Concentration
LYOPH
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Reconstitute with 50 ul endotoxin-free water (1mg/ml after reconstitution)

Alternate Names for Recombinant Rat Adiponectin/Acrp30 FLAG Protein

  • ACDC
  • Acrp30
  • ACRP30ADPN
  • adipocyte, C1Q and collagen domain containing
  • Adiponectin
  • adiponectin, C1Q and collagen domain containing
  • AdipoQ
  • ADIPQTL1
  • ApM1
  • apM-1
  • APM1APM-1
  • C1q and collagen domain-containing protein
  • GBP28
  • GBP28apM1
  • Gelatin-binding protein

Background

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Rat
Adiponectin/Acrp30 FL ...
NBP3-18139
Recombinant Rat Adiponectin/Acrp30 FLAG Protein
Species: Rt
Applications: WB, Bioactivity

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Bioinformatics

Gene Symbol ADIPOQ
Uniprot