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Recombinant Human Adenylate Kinase 1 His Protein

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SDS-Page: Recombinant Human Adenylate Kinase 1 Protein [NBP1-50855]
SDS-Page: Recombinant Human Adenylate Kinase 1 Protein [NBP1-50855]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Enzyme Activity
Concentration
1 mg/ml

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Recombinant Human Adenylate Kinase 1 His Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-194 of Human Adenylate Kinase 1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK
Details of Functionality
Specific activity > 600 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
AK1
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • SDS-Page
Theoretical MW
23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 7.5), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human Adenylate Kinase 1 His Protein

  • Adenylate Kinase 1
  • adenylate kinase isoenzyme 1
  • AK 1
  • AK1
  • ATP-AMP transphosphorylase 1
  • EC 2.7.4
  • EC 2.7.4.3
  • EC 2.7.4.6
  • Myokinase

Background

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol AK1