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Adenylate Kinase 1 Antibody (OTI4A1)

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Western Blot: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] Analysis of extracts (35ug) from 9 different cell lines by using anti-Adenylate Kinase 1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] - Immunofluorescent staining of HT29 cells using anti-Adenylate Kinase 1 mouse monoclonal antibody.
Flow Cytometry: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Adenylate Kinase 1 ...read more
Western Blot: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adenylate Kinase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI4A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.89 mg/ml

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Adenylate Kinase 1 Antibody (OTI4A1) Summary

Immunogen
Full length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens adenylate kinase 1 (AK1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000-2000
Theoretical MW
21.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.89 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Adenylate Kinase 1 Antibody (OTI4A1)

  • Adenylate Kinase 1
  • adenylate kinase isoenzyme 1
  • AK 1
  • AK1
  • ATP-AMP transphosphorylase 1
  • EC 2.7.4
  • EC 2.7.4.3
  • EC 2.7.4.6
  • Myokinase

Background

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AK1
Entrez