ADAM28 Antibody (1004266) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 aa extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3).  ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4 beta 1(9).  R&D Systems in-house testing also indicates that ADAM28 cleaves IGFBP-3.

1. Roberts, C.M. et al. (1999) J. Biol. Chem. 274:29251.
2. McGinn, O.J. et al. (2011) Cell Biol. Int. 35:1043.
3. Howard, L. et al. (2000) Biochem. J. 348:21.
4. Mochizuki, S. and Okada Y, (2007) Cancer Sci. 98:621.
5. Schlondorff, J. et al. (1999) J. Cell Sci. 112:3603.
6. Blobel, C.P. (1997) Cell 90:589.
7. Mochizuki S. and Okada Y. (2009) Curr. Pharm. Des. 15:2349.
8. Edwards, L. et al. (2008) Molecular Aspects of Medicine 29:258.
9. Bridges, L. et al. (2002) J. Biol. Chem. 277:3784.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Array MAB93252

• ADAM28 Antibodies
• ADAM28 ELISA Kits
• ADAM28 Lysates
• ADAM28 Protein
• ADAM28 Proteins and Enzyme