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ADAM15 Antibody (23G9) [Unconjugated]

 (5 Publications)
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ADAM15 was detected in immersion fixed MCF-7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ADAM15 was detected in immersion fixed MCF-7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at 4 °C. Tissue was stained ...read more
ADAM15 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in MOLT‑4 human acute lymphoblastic leukemia cells (Negative) using Mouse Anti-Human ADAM15 Ectodomain Monoclonal ...read more
miR-147b down-regulates cell surface expression of ADAM15 in endothelial cells.At 48 h after transfection, live HUVECs were labeled with a monoclonal anti-ADAM15 ectodomain antibody and a secondary Cy3 conjugate. Cell ...read more
miR-147b down-regulates cell surface expression of ADAM15 in endothelial cells.At 48 h after transfection, live HUVECs were labeled with a monoclonal anti-ADAM15 ectodomain antibody and a secondary Cy3 conjugate. Cell ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, CyTOF-ready, Flow, ICC/IF
Clone
23G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC935U-100UG
Alexa Fluor 405
IC935V-100UG
Alexa Fluor 488
IC935G-100UG
Alexa Fluor 532
FAB935X
Alexa Fluor 594
IC935T-100UG
Alexa Fluor 647
IC935R-100UG
Alexa Fluor 700
IC935N-100UG
Alexa Fluor 750
IC935S-100UG
Allophycocyanin
FAB935A
Biotin
FAB935B
CoraFluor 1
FAB935CL1
DyLight 350
FAB935D
DyLight 405
FAB935E
DyLight 488
FAB935K
DyLight 550
FAB935L
DyLight 594
FAB935M
DyLight 650
FAB935W
DyLight 680
FAB935Y
DyLight 755
FAB935Z
FITC
FAB935F
HRP
FAB935H
Janelia Fluor 525
FAB935JF525
Janelia Fluor 549
FAB935I
Janelia Fluor 585
FAB935JF585
Janelia Fluor 635
FAB935JF635
Janelia Fluor 646
FAB935J
Janelia Fluor 669
FAB935JF669
PE
FAB935P
PerCP
FAB935C
mFluor Violet 450 SE
FAB935MFV450
mFluor Violet 500 SE
FAB935MFV500
mFluor Violet 610 SE
FAB935MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB935-SP
Carrier Free
MAB935-500
Carrier Free
IC935U-100UG
Carrier Free
IC935N-100UG
Carrier Free
IC935G-100UG
Carrier Free
IC935T-100UG
Carrier Free
IC935S-100UG
Carrier Free
IC935V-100UG
Carrier Free
IC935R-100UG
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ADAM15 Antibody (23G9) [Unconjugated] Summary

Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15
Asp207-Thr696
Accession # Q13444
Specificity
Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross‑reactivity with recombinant mouse (rm) ADAM15 but does not cross‑react with rhADAM8, 9, 17, 28, rmADAM9, or 10.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ADAM15
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB935 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM15 Antibody (23G9) [Unconjugated]

  • ADAM 15
  • ADAM metallopeptidase domain 15
  • ADAM15
  • disintegrin-like, and cysteine-rich protein 15
  • EC 3.4.24
  • MDC15
  • MDC-15
  • Metargidin

Background

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ADAM15 Antibody
(23G9) [Unconjugated]
MAB935
ADAM15 Antibody (23G9) [Unconjugated]
Species: Hu
Applications: WB, IHC, IP, CyTOF-ready, Flow, ICC/IF

Publications for ADAM15 Antibody (MAB935)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


Filter By Application
Flow Cytometry
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB935 Applications Species
Takehiko Ichikawa, Dong Wang, Keisuke Miyazawa, Kazuki Miyata, Masanobu Oshima, Takeshi Fukuma Chemical fixation creates nanoscale clusters on the cell surface by aggregating membrane proteins Communications Biology 5/20/2022 [PMID: 35595960]
LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer Cell Rep, 2020-10-13;33(2):108253. 10/13/2020 [PMID: 33053339]
Chatterjee V, Beard R, Reynolds J, Haines R, Guo M, Rubin M, Guido J, Wu M, Yuan S MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression. PLoS ONE, 2014-10-15;9(10):e110286. 2014-10-15 [PMID: 25333931] (Flow Cytometry, Human) Flow Cytometry Human
Ungerer C, Doberstein K, Burger C, Hardt K, Boehncke WH, Bohm B, Pfeilschifter J, Dummer R, Mihic-Probst D, Gutwein P ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma. Biochem. Biophys. Res. Commun., 2010-09-17;401(3):363-9. 2010-09-17 [PMID: 20851104] (Flow Cytometry, Human) Flow Cytometry Human
Bohm BB, Aigner T, Roy B, Brodie TA, Blobel CP, Burkhardt H Homeostatic effects of the metalloproteinase disintegrin ADAM15 in degenerative cartilage remodeling. Arthritis Rheum., 2005-04-01;52(4):1100-9. 2005-04-01 [PMID: 15818704] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol ADAM15
Uniprot