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ADAM22 Antibody (498805) [Unconjugated]

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Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, Jurkat human acute T cell leukemia cell line (negative control), and HepG2 human hepatocellular carcinoma cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ADAM22 Monoclonal Antibody (Catalog # MAB49081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for ADAM22 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, Jurkat human acute T cell leukemia cell line (negative control), and HepG2 human hepatocellular carcinoma cell line ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
498805
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB49081-SP
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ADAM22 Antibody (498805) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAM22
Gly26-His528
Accession # Q9P0K1
Specificity
Detects human ADAM22 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ADAM8, 9, 10, 12, 15, 19, 23, 32, or 33 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ADAM22
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM22 Antibody (498805) [Unconjugated]

  • ADAM 22
  • ADAM metallopeptidase domain 22
  • ADAM22
  • MDC2
  • metalloproteinase-disintegrin ADAM22-3
  • metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
  • MGC149832

Background

Human ADAM22 is a member of the ADAM (a disintegrin and metalloprotease domain) family of metalloproteases. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins. They have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, such as fertilization, muscle development, and neurogenesis. ADAM22 is highly expressed in the brain and may function as an integrin ligand in the brain. The amino acid sequence of human ADAM22 is 100%, 96% and 92% identical to that of chimpanzee, dog and mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ADAM22 Antibody
(498805) [Unconjugate
MAB49081
ADAM22 Antibody (498805) [Unconjugated]
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Bioinformatics

Gene Symbol ADAM22
Uniprot