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ACTR1B Antibody

Images

 
Western Blot: ACTR1B Antibody [NB100-1424] - (1ug/ml) Analysis human heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry: ACTR1B Antibody [NB100-1424] - (3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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ACTR1B Antibody Summary

Immunogen
Peptide with sequence C-YEEDGSRAIHRKT corresponding to C-Terminus according to NP_005726.1.
Localization
Cytoplasmic
Specificity
This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACTR1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.5 - 2 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in human heart and brain lysates (predicted MW of 43 kDa band according to NP_005726). IHC-P: Human uterus shows fibrillar staining of cytoplasm in endometrial cells.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ACTR1B Overexpression Lysate
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-1424.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ACTR1B Antibody

  • Actin-related protein 1B
  • ARP1 (actin-related protein 1, yeast) homolog B (centractin beta)
  • ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
  • ARP1BPC3
  • centractin beta
  • CTRN2beta-centractin

Background

In addition to conventional actins, there are several actin-related proteins. The ACTR1B protein and the ACTR1A protein are of equal length, and they share 90% amino acid identity and 96% amino acid similarity. ACTR1B is expressed in the cytosol as part of the dynactin complex. The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1B appears to be ivolved in dynein-driven vesicle movement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACTR1B Antibody (NB100-1424)(1)

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Bioinformatics

Gene Symbol ACTR1B
Entrez
Uniprot