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Acrosin Antibody (ACR-2) [mFluor Violet 610 SE]

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Acrosin Antibody (ACR-2) [mFluor Violet 610 SE] [NB500-419MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission ...read more

Product Details

Summary
Reactivity Po, Bv(-), Ca(-), Hu(-)Species Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
ACR-2
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 610 SE

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Acrosin Antibody (ACR-2) [mFluor Violet 610 SE] Summary

Immunogen
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Specificity
The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACR
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Acrosin Antibody (ACR-2) [mFluor Violet 610 SE]

  • acrosin
  • ACRS
  • preproacrosin
  • proacrosin

Background

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB500-419MFV610
Species: Po, Bv(-), Ca(-), Hu(-)
Applications: WB, Flow, ICC/IF, CyTOF-ready

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACR