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Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free

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Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.
Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.
Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.
Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Western Blot 1:2000
Theoretical MW
85.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free

  • aconitase 2, mitochondrial
  • Aconitase
  • aconitate hydratase, mitochondrial
  • ACONM
  • Citrate hydro-lyase
  • EC 4.2.1
  • EC 4.2.1.3
  • MGC20605
  • MGC33908

Background

Aconitase 2 is encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACO2