![Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in U251 Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-HR2-Immunocytochemistry-Immunofluorescence-NB300-528-img0006.jpg "Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in U251 Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
| Reactivity | Hu, Bv, Fe, Rb, Mu(-), Rt(-)Species Glossary |
| Applications | ELISA, ICC/IF, IHC, IP |
| Clone | HR2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified human cerebellar acetylcholinesterase. |
| Localization | Cell Membrane and Secreted |
| Marker | Early Neuronal Development Marker |
| Specificity | This antibody does not detect butyrylcholinesterase (BChE). |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ACHE |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IHC: Staining of AChE in human brain samples results in staining of nerve fibers and terminals. Cannot be used in WB to detect AChE. Not suitable for Flow. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NB300-528 | Applications | Species |
|---|---|---|
| Zeineh MM, Chen Y, Kitzler HH et al. Activated iron-containing microglia in the human hippocampus identified by magnetic resonance imaging in Alzheimer disease. Neurobiol Aging 2015-09-01 [PMID: 26190634] (Human) | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Acetylcholinesterase/ACHE Antibody (NB300-528)Find related products by research area.
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HIV-associated neurocognitive disorders involve extracellular Nef-induced modification of lipid rafts and redistribution of Alzheimer’s disease-related proteins Jamshed Arslan, Pharm D, PhD Cholesterol is an essential part of animal cell membranes. Cholesterol-rich lipid rafts maintain the fluidity and protein trafficking of plasma membranes. Cellular ABCA1 protein moves cho... Read full blog post. |
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![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Cerebellum tissue.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-HR2-Immunohistochemistry-Paraffin-NB300-528-img0008.jpg "Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Cerebellum tissue.")
![Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in Hela Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-HR2-Immunocytochemistry-Immunofluorescence-NB300-528-img0004.jpg "Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in Hela Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in Neuro-2a Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-HR2-Immunocytochemistry-Immunofluorescence-NB300-528-img0005.jpg "Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in Neuro-2a Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Rectum tissue.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-HR2-Immunohistochemistry-Paraffin-NB300-528-img0007.jpg "Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Rectum tissue.")
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Brain tissue.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-HR2-Immunohistochemistry-Paraffin-NB300-528-img0009.jpg "Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Brain tissue.")
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