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ACAA1 Antibody

Images

 
Independent Antibodies: Western Blot: ACAA1 Antibody [NBP1-86128] - Analysis using Anti-ACAA1 antibody NBP1-86128 (A) shows similar pattern to independent antibody NBP1-85786 (B).
Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody [NBP1-86128] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Immunohistochemistry-Paraffin: ACAA1 Antibody [NBP1-86128] - Staining of human cerebral cortex.
Western Blot: ACAA1 Antibody [NBP1-86128] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat ...read more
Independent Antibodies: Western Blot: ACAA1 Antibody [NBP1-86128] - Staining of human cerebral cortex, colon, kidney and liver using Anti-ACAA1 antibody NBP1-86128 (A) shows similar protein distribution across ...read more
Immunohistochemistry-Paraffin: ACAA1 Antibody [NBP1-86128] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ACAA1 Antibody [NBP1-86128] - Staining of human liver.
Immunohistochemistry-Paraffin: ACAA1 Antibody [NBP1-86128] - Staining of human colon.
Immunohistochemistry-Paraffin: ACAA1 Antibody [NBP1-86128] - Staining of human kidney.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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ACAA1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GNSSQVSDGAAAILLARRSKAEELGLPILGVLRSYAVVGVPPDIMGIGPAYAIPVA
Marker
Peroxisome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACAA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ACAA1 Protein (NBP1-86128PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ACAA1 Antibody

  • acetyl-CoA acyltransferase 1
  • Acetyl-CoA acyltransferase
  • EC 2.3.1
  • peroxisomal

Background

Acetyl-Coenzyme A acyltransferase (ACAA1) is an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ACAA1 Antibody (NBP1-86128) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACAA1