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Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

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ELISA: Human 5-Lipoxygenase ELISA Kit (Chemiluminescence) [NBP2-66366] - Samples were spiked with high concentrations of Human 5-Lipoxygenase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human 5-Lipoxygenase ELISA Kit (Chemiluminescence) [NBP2-66366] - Samples were spiked with high concentrations of Human 5-Lipoxygenase and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human 5-Lipoxygenase ELISA Kit (Chemiluminescence) [NBP2-66366] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.50-4000 pg/mL
Sensitivity
37.50 pg/mL

Order Details

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Human 5-Lipoxygenase ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human 5-LO in samples. No significant cross-reactivity or interference between Human 5-LO and analogues was observed.
Standard Curve Range
62.50-4000 pg/mL
Sensitivity
37.50 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.46%
Intra-Assay
CV% < 9.46%
Spike Recovery
84-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
ALOX5

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

  • 5Lipoxygenase
  • 5-Lipoxygenase
  • 5-LO
  • 5-LOX
  • 5LPG
  • ALOX5
  • arachidonate 5-lipoxygenase
  • arachidonic 5-lipoxygenase alpha-10 isoform
  • arachidonic 5-lipoxygenase delta-10-13 isoform
  • arachidonic 5-lipoxygenase delta-13 isoform
  • arachidonic 5-lipoxygenase delta-p10 isoform
  • arachidonic acid 5-lipoxygenase
  • EC 1.13.11
  • EC 1.13.11.34
  • leukotriene A4 synthase
  • LOG5
  • LOX5A
  • MGC163204

Background

5 Lipoxygenase encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs

  Human 5-Lipoxygenase ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol ALOX5