SARS Nucleocapsid Protein Antibodies
Coronaviruses are RNA viruses and seven Coronavirus strains are known to cause human infection including SARS-COV-2 (COVID-19). The SARS-COV Nucleocapsid (N) protein, with a theoretical molecular weight of 46 kDa, is highly immunogenic and expressed primarily in the cytoplasm. We offer SARS-COV Nucleocapsid (N) protein antibodies that have been cited in 20+ publications. RNA sequences of SARS-CoV-2 and SARS-CoV share ~80% identity and some SARS Nucleocapsid antibodies will detect both SARS-COV and SARS-COV-2 antigens. View SARS Nucleocapsid antibody datasheets for more information on cross-reactivity. Our extensive catalog includes Coronavirus Nucleocapsid polyclonal antibodies, monoclonal antibodies, and fluorescently-conjugated SARS-COV Nucleocapsid Protein antibodies. Validated research applications for SARS Nucleocapsid antibodies include ELISA, ICC, IHC, IHC-Paraffin, Immunofluorescence, and Western Blot. Choose the anti-SARS Coronavirus Nucleocapsid Protein antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each SARS Nucleocapsid (N) antibody is fully covered by our Guarantee+.
Alternate Names for SARS Nucleocapsid Protein Antibodies
anti-SARS Nucleocapsid Protein antibody, anti-Coronavirus Nucleocapsid Protein antibody, anti-N antibody, anti-SARS antibody, anti-N protein antibody, anti-N structural protein antibody, anti-NC antibody, anti-Nucleocapsid protein antibody, anti-SARS coronavirus N protein antibody, anti-SARS coronavirus nucleocapsid protein antibody, anti-SARS CoV antibody, anti-SARS CoV N protein antibody, anti-SARS CoV nucleocapsid protein antibody, anti-SARS N protein antibody, anti-SARSCoV antibody, anti-SARSCoV N protein antibody, anti-SARSCoV nucleocapsid protein antibody, anti-Severe acute respiratory syndrome antibody
15 Results for "sars-nucleocapsid-protein" in Primary Antibodies
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0005.jpg "Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316")
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56576] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S (848561); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Dual-RNAscope-ISH-IHC-NB100-56576-img0006.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Characteristics of 2B4 cells clonally derived from human bronchial epithelial Calu-3 cells. Finally, 2B4 cells (passage 6) were infected with SARS-CoV (MOI = 0.1) for 24, 48, and 72 hrs before being fixed with 4% paraformaldehyde for monitoring the morphological changes of infected cells, as visualized by the expression of SARS-CoV NP protein (red) by using the standard IHC (F). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008729) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0003.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - Western blot shows recombinant SARS-CoV-2 Nucleocapsid protein. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-SARS-CoV-2 Nucleocapsid Polyclonal Antibody (Catalog # NB100-56576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for SARS-CoV-2 Nucleocapsid at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56576-img0007.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56576-img0002.jpg)
![Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Simple-Western-NB100-56683-img0004.jpg "Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56683] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S [848561]); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Dual-RNAscope-ISH-IHC-NB100-56683-img0005.jpg)
![Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) only in the SARS-CoV-2 lysate using 25 ug/mL of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Note: some reactivity observed with FL Std 230. SARS-CoV-2 lysate courtesy of University of Maryland.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Simple-Western-NB100-56683-img0003.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56683] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody at a 1:2000 dilution.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56683-img0002.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56049] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56049-img0002.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56049] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.")
![Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NBP2-44205-img0004.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44205-img0003.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NBP2-44205-img0005.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: SARS CoV Nucleocapsid (N) Protein [20 ng]. Primary Antibody: Anti-SARS CoV Nucleocapsid (N) Protein Antibody at 1.0 ug/mL overnight at 2-8 degrees C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40000 for 30 mins at RT. Predicted MW: ~46 kDa. Observed MW: ~48 kDa.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NBP2-44205-img0001.jpg)
![ELISA: SARS Nucleocapsid Protein Antibody [NBP2-44205] - ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein. Each well was coated in duplicate with 0.5ug/mL of SARS CoV-1 [Red Line], SARS CoV-2 Nc [Green Line], MERS CoV N [Blue Line]. The starting dilution of antibody was 2 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-1N IC50: 11ng/mL, SARS CoV-2N IC50: 15 ng/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-ELISA-NBP2-44205-img0006.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56050-img0002.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.")
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (001) [NBP2-90988] - 1:2000 dilution. Lane A: SARS-CoV-2 NP Protein (30ng). Lane B: SARS-CoV NP Protein (5ng). Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng). Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng). Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-001-Western-Blot-NBP2-90988-img0001.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody (001) [NBP2-90988] - 1:2000 dilution. Lane A: SARS-CoV-2 NP Protein (30ng). Lane B: SARS-CoV NP Protein (5ng). Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng). Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng). Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.")
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARSNucleocapsid Rabbit Mab,then a HRPconjugated second step antibody](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-001-Immunocytochemistry-Immunofluorescence-NBP2-90988-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Rabbit Mab,then a Alexa Fluor®488-conjugated second step antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-001-Immunocytochemistry-Immunofluorescence-NBP2-90988-img0002.jpg)
![ELISA: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Nucleocapsid-His recombinant Protein,Biotinylated on SA Biosensor, can bind SARSCoV/ SARS-CoV-2 Nucleocapsid Antibody, Rabbit MAb with an affinity constant of 0.02 nM as determined by Octet RED System.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-001-ELISA-NBP2-90988-img0004.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody (05) [NBP3-06413] - Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein (30ng)Lane B: SARS-CoV NP Protein (5ng)Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)Secondary: Goat Anti-Mouse IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-05-Western-Blot-NBP3-06413-img0001.jpg "Western Blot: SARS Nucleocapsid Protein Antibody (05) [NBP3-06413] - Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein (30ng)Lane B: SARS-CoV NP Protein (5ng)Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)Secondary: Goat Anti-Mouse IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.")
![Western Blot: SARS Nucleocapsid Protein Antibody (14B3D) [NBP3-07063] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid protein, Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [14B3D], NBP3-07063, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-14B3D-Western-Blot-NBP3-07063-img0001.jpg "Western Blot: SARS Nucleocapsid Protein Antibody (14B3D) [NBP3-07063] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid protein, Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [14B3D], NBP3-07063, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.")
![Western Blot: SARS Nucleocapsid Protein Antibody (8G8A) [NBP3-07064] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid Protein and Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [8G8A], NBP3-07064, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-8G8A-Western-Blot-NBP3-07064-img0001.jpg "Western Blot: SARS Nucleocapsid Protein Antibody (8G8A) [NBP3-07064] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid Protein and Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [8G8A], NBP3-07064, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.")