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SARS Nucleocapsid Protein Antibody (001) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity V, VSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
001
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 647

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SARS Nucleocapsid Protein Antibody (001) [Alexa Fluor® 647] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant SARS-CoV Nucleoprotein/NP Protein
Specificity
Has cross-reactivity in ELISA and WB with:
SARS-CoV-2 Nucleocapsid Protein

Has cross-reactivity in ELISA with:
SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein
SARS-CoV-2 Nucleocapsid Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) Protein
SARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) Protein
SARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) Protein
SARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) Protein
SARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) Protein
SARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) Protein
SARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) Protein
SARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) Protein
SARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) Protein
SARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) Protein

No cross-reactivity in ELISA with:
MERS-CoV Nucleocapsid protein
HCoV-229E Nucleocapsid protein
HCoV-NL63 Nucleocapsid protein
HCoV-HKU1(isolate N5) Nucleocapsid protein
HCoV-OC43 Nucleocapsid protein
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
N
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

This antibody has cross-reactivity in ELISA and WB with:
SARS-CoV-2 Nucleocapsid Protein

No cross-reactivity in ELISA with:
MERS-CoV Nucleoprotein protein
HCoV-229E Nucleoprotein protein
HCoV-NL63 Nucleoprotein protein
HCoV-HKU1(isolate N5) Nucleoprotein protein
HCoV-OC43 Nucleoprotein

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SARS Nucleocapsid Protein Antibody (001) [Alexa Fluor® 647]

  • N protein
  • N structural protein
  • N
  • NC
  • Nucleocapsid protein
  • Nucleoprotein
  • Protein N
  • SARS coronavirus N protein
  • SARS coronavirus nucleocapsid protein
  • SARS CoV N protein
  • SARS CoV nucleocapsid protein
  • SARS CoV
  • SARS N protein
  • SARS Nucleoprotein
  • SARSCoV N protein
  • SARSCoV nucleocapsid protein
  • SARSCoV
  • Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SARS Nucleocapsid Protein Antibody (NBP2-90988AF647) (0)

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol N