GRP78/HSPA5 Antibodies
We offer GRP78/HSPA5 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each GRP78/HSPA5 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GRP78/HSPA5 Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Fungi, Hamster, Human, Mouse, Primate, Rabbit, Rat, Sheep, Xenopus. Use the list below to choose the GRP78/HSPA5 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GRP78/HSPA5 polyclonal antibodies and browse our GRP78/HSPA5 monoclonal antibody catalog.
Alternate Names for GRP78/HSPA5 Antibodies
anti-GRP78/HSPA5 antibody, anti-HSPA5 antibody, anti-78 kDa glucose-regulated protein antibody, anti-BiP antibody, anti-Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody, anti-FLJ26106 antibody, anti-GRP-78 antibody, anti-GRP78BIP antibody, anti-Heat shock 70 kDa protein 5 antibody, anti-heat shock 70kD protein 5 (glucose-regulated protein, 78kD) antibody, anti-heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antibody, anti-Immunoglobulin heavy chain-binding protein antibody, anti-MIF2 antibody
21 Results for "grp78-hspa5" in Primary Antibodies
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06274-img0006.jpg "Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - C. elegans granulins impair organismal fitness and resistance to ER stress. Total worm lysates from synchronized day 1 adult granulin-expressing animals were immunoblotted with an anti-GRP78/HSPA5 antibody (3 biological replicates). Anti-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1008295), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06274-img0016.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis of MAM-associated protein levels in control and patients' fibroblasts.(A) Equal amounts from one control and the five patients were loaded (50 ug of total cell lysates) and subjected to Western Blot with anti-Grp75, anti-sigma-1R and anti-Mfn2 antibodies. We used anti-GAPDH antibody to ensure equal amounts of protein loaded in each lane. This result is representative of three independent experiments. PLoS One. 2016 Mar 9;11(3):e0150357. doi: 10.1371/journal.pone.0150357](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06274-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - NIH-3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 2 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06274-img0013.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-GPR78 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06274-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-GRP78/HSPA5 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06274-img0015.jpg)
![Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on NIH3T3 cells with GRP78/HSPA5 Antibody NBP1-06274AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0014.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Detection of Bip/Grp78 on HeLa whole cell extracts using NBP1-06274.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06274-img0004.jpg)
![Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis using Alexa Fluor (R) 488 conjugate of NBP1-06274. An intracellular stain was performed on HeLa cells with GPR78/HSPA5 antibody NBP1-06274 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0007.jpg)
![Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on Jurkat cells with Alexa Fluor 647 conjugate of GPR78/HSPA5 antibody NBP1-06274AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0008.jpg)
![Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Flow-Cytometry-NBP1-06274-img0012.jpg)
![Western Blot: GRP78/HSPA5 Antibody - BSA Free [NBP1-06274] - Hotair promotes the expression of GRP78 & PD-L1, mediates the proliferation, & helps to regulate the T cell-mediated immune responses in Tu212 & Hep-2 cells. Western blot analysis of GRP78 & PD-L1 after mi-NC or has-miR-30a-5p & vehicle or Hotair transfection in Hep-2 cells (a). The relative expression level of GRP78 & PD-L1 in Hep-2 cells (b). (c–e) The cell viability, apoptosis, & colony formation after mi-NC or has-miR-30a-5p & vehicle or Hotair transfection in Hep-2 cells. (f–h) T cell killing assay of target Tu212 & Hep-2 cells cocultured with PBMC, & ELISA detects the concentration of cytokines (g) IFN-gamma & (h) IL-2. The targeted Tu212 & Hep-2 cells after 24 h transfected with mi-NC or has-miR-30a-5p & vehicle or Hotair were cocultured with PBMC, & the cytotoxicity was measured by the LDH release assay. Data are mean ± SD of at least three independent experiments; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35419461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06274_rabbit-polyclonal-grp78-hspa5-antibody-310202416175290.jpg)
![Western Blot: GRP78/HSPA5 Antibody - BSA Free [NBP1-06274] - Analysis of protein levels involved in ER stress & Ca2+ homeostasis & processing of mRNA XBP1 in control & patients-derived fibroblasts.(A) Equal amounts from controls & patients were loaded (50 μg of total cell lysates) & subjected to Western Blot with anti-Herp, anti-Grp78 & anti-IP3R1 antibodies. We used anti-Hsp60 antibody to ensure equal amounts of protein loaded in each lane. This result is representative of three independent experiments. Protein quantification was performed by laser densitometry. The ratios between proteins/Hsp60 for each cell line were calculated to determine the expression fold-change relative to control. (B) Data represent mean ± standard deviation of three independent experiments. (C) Equal amounts from controls were loaded (50 μg of total cell lysates) & subjected to Western Blot with anti-Herp & anti-Grp78 antibodies. We used anti-GAPDH antibody to ensure equal amounts of protein loaded in each lane. This result is representative of two independent experiments. (D) Equal amounts from control & patients were loaded (50 μg of total cell lysates) & subjected to Western Blot with anti-phospho-PERK antibody. We used anti-GAPDH antibody to ensure equal amounts of protein loaded in each lane. This result is representative of two independent experiments. (E) RT-PCR analysis of the processing of mRNA XBP1 transcription factor. Tm: tunicamycin; u: XBP1 unspliced form; s: XBP1 spliced form. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150357), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06274_rabbit-polyclonal-grp78-hspa5-antibody-310202416182615.jpg)
![Western Blot: GRP78/HSPA5 Antibody - BSA Free [NBP1-06274] - UPR Components Activated in A beta -Expressing Flies Are Induced Even Further by Glut1 Overexpression(A) Western blot of eIF2 phosphorylation levels in heads of A beta - & A beta Glut1-expressing flies (+RU) & in controls (-RU), showing no significant difference. Bottom: plotted as means ± SEM (n = 3). Top: a representative gel from the same samples.(B) Grp78 mRNA levels in heads of 18-day-old flies expressing A beta or A beta Glut1 in neurons (+RU) & uninduced controls (-RU), measured by qPCR (relative to eIF1A), plotted as means ± SEM. Genotypes: UAS A beta ; elavGS, UAS A beta /UAS Glut1; elavGS.(C) Quantification of GFP fluorescence in fly brains expressing an Xbp1GFP splicing reporter, plotted as means ± SEM (n = 6–13). Genotypes: elavGS/UAS-Xbp1GFP, UAS A beta ; elavGS/UAS-Xbp1GFP, UAS A beta /UAS Glut1; elavGS/UAS-Xbp1GFP.(D) Spliced Xbp1 mRNA levels in heads of 18-day-old flies expressing A beta or A beta Glut1 in neurons (+RU) & uninduced controls (-RU), measured by qPCR (relative to eIF1A), plotted as means ± SEM(E) Western blot of Grp78 in 14-day-old flies of the same genotypes, plotted below as means ± SEM (n = 6–16). The image is a representative gel of the same samples. Genotypes: UAS A beta ; elavGS, UAS A beta /UAS Glut1; elavGS.∗p ≤ 0.05; ∗∗p ≤ 0.01, by ANOVA. See also Figure S2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27524482), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06274_rabbit-polyclonal-grp78-hspa5-antibody-310202416163730.jpg)
![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody - BSA Free [NBP1-06274] - C. elegans granulins impair organismal fitness & resistance to ER stress.(A) Wild-type (N2) & pgrn-1(-) animals with & without granulin expression were subjected to ER stress with tunicamycin (5 μg / ml). The fraction developing to L4 stage was quantified (n = 50, 3 biological replicates). (B) Wild-type (N2) & pgrn-1(-) animals with & without C. elegans progranulin over-expression (OE) were subjected to ER stress with tunicamycin (5 μg / ml). The fraction developing to L4 stage was quantified (n = 50, 3 biological replicates). (C) Total worm lysates from synchronized day 1 adult granulin-expressing animals were immunoblotted with an anti-HSP-4/BiP antibody (3 biological replicates). Anti-actin was used as a loading control. (D) Wild-type & pgrn-1(-) animals with & without granulin expression were staged as embryos. Animals were scored for development to L4 stage (n = 50, 12 biological replicates). (E) Measurement of body length at day 1 adulthood (n = 12). (F) Measurement of short-term associative learning (three biological replicates). The glutamate receptor mutant nmr-1(ak4) was used as a positive control. Throughout, error bars show mean ± SEM, one or two-way ANOVA with post-hoc Tukey multiple comparisons test. Comparisons are to wild-type unless otherwise indicated (*P<0.05, ***P<0.001, ****P<0.0001, ns = not significant, wt = wild-type). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31398187), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06274_rabbit-polyclonal-grp78-hspa5-antibody-310202416173547.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB300-520] - GRP78 on indicated cell extracts using NB300-520.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB300-520-img0004.jpg "Western Blot: GRP78/HSPA5 Antibody [NB300-520] - GRP78 on indicated cell extracts using NB300-520.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NB300-520] - GRP78/BIP antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NB300-520-img0005.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NB300-520] - Lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NB300-520-img0006.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB100-56411-img0004.jpg "Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.")
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in formalin-fixed, paraffin-embedded mouse liver tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NB100-56411-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in mouse liver tissue enriched using Endoplasmic Reticulum Enrichment Kit and 1 ug/ml of NB100-56411 and 0.25 ug/ml of anti-GAPDH loading control. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB100-56411-img0003.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NB100-56411] - Simple Western lane view shows a specific band for GRP78/HSPA5 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NB100-56411-img0005.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-16749-img0020.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16749-img0018.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat duodenum. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0016.jpg)
![Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/GRP78-Antibody-Western-Blot-NBP2-16749-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 7.5% SDS-PAGE Grp78 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-16749-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-16749-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16749-img0017.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0005.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0006.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse testis. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0013.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat liver. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0014.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse intestine. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0015.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - GRP78/HSPA5 proteins were measured by immunoblotting in epithelioid (EPI BAP1+ UPN7), biphasic (BIP BAP1- UPN16) and sarcomatous (BAP1+ UPN22) MPM cells. Tubulin was used as a loading control. The figure is representative of 1 out of 3 experiments with similar results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35197103/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-25520231539516.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-1610202419405722.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06277-img0005.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06277] - BIP/Grp78 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06277-img0006.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0008.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band detected for GRP78 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0007.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining in human placenta and skeletal muscle tissues.. Corresponding HSPA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining in human placenta and skeletal muscle tissues.. Corresponding HSPA5 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: GRP78/HSPA5 Antibody [NBP1-89969] - Analysis using Anti-HSPA5 antibody NBP1-89969 (A) shows similar pattern to independent antibody NBP1-89968 (B).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-89969-img0014.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0013.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-89969] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-89969-img0007.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0009.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0011.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human rectum shows strong cytoplasmic positivity in glandular and lymphoid cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0012.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody diluted at a dilution of 1:10000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-42848-img0009.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody diluted at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-42848] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hspa5 protein stained by Hspa5 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42848-img0006.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Paraffin-embedded rat brain. Hspa5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-42848-img0008.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of 30 ug whole cell lysate. A: NIH-3T-3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 7.5% SDS PAGE diluted at 1:10000..](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-42848-img0003.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of A. 50 ug Rat heart lysate/extract B. 50 ug Rat Lung lysate/extract C. 50 ug Rat Liver lysate/extract D. 50 ug Rat kidney lysate/extract 7.5 % SDS-PAGE Hspa5 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-42848-img0004.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of gastric carcinoma. Hspa5 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-42848-img0001.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Paraffin-embedded mouse liver. Hspa5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-42848-img0007.jpg)
![Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Western-Blot-NBP2-59692-img0002.jpg "Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Mouse fibroblast cell line (NIH 3T3). Fixed in 4% Formaldehyde for 15 min at RT. Primary antibody at 1:100 for 60 min at RT. Secondary antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstaied with DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Immunocytochemistry-Immunofluorescence-NBP2-59692-img0003.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-54318-img0008.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-54318] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54318-img0006.jpg)
![Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Western-Blot-NBP2-12888-img0003.jpg "Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0005.jpg)