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PIST Antibody

Images

 
Genetic Strategies: Western Blot: PIST Antibody [NBP1-88223] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented
Immunocytochemistry/ Immunofluorescence: PIST Antibody [NBP1-88223] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: PIST Antibody [NBP1-88223] - Staining of human stomach shows strong cytoplasmic and membrane positivity in glandular cells.
Western Blot: PIST Antibody [NBP1-88223] - Analysis in human cell line HepG2.
Genetic Strategies: Western Blot: PIST Antibody [NBP1-88223] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1, using Anti-GOPC antibody. Remaining relative intensity is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

PIST Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IEFEVVYVAPEVDSDDENVEYEDESGHRYRLYLDELEGGGNPGASCKDTSGEIKVLQGFNKKAVTDTHENGDLGTASETPLDDGASKLDDLHTLY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOPC
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PIST Protein (NBP1-88223PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PIST Antibody

  • CALPDZ protein interacting specifically with TC10
  • CFTR-associated ligand
  • dJ94G16.2
  • FIGdJ94G16.2 PIST
  • Fused in glioblastoma
  • golgi-associated PDZ and coiled-coil motif containing
  • Golgi-associated PDZ and coiled-coil motif-containing protein
  • GOPC1
  • PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
  • PISTGolgi associated PDZ and coiled-coil motif containing protein

Background

PIST is a PDZ domain-containing Golgi protein. PDZ domains contain approximately 90 amino acids and bind the extreme Cterminus of proteins in a sequence-specific manner.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PIST Antibody (NBP1-88223) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for PIST Antibody (NBP1-88223)

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Blogs on PIST

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Contact Information

Product PDFs

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Bioinformatics

Gene Symbol GOPC