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![Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-39D-Western-Blot-NBP1-43299-img0004.jpg "Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.")
![Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-288202412461675.jpg "Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32908) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29820241492635.jpg)
![Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunocytochemistry analysis of NK-92 (positive) and K-562 (negative) labeling T-bet/TBX21 with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-292024119237.jpg)
![Flow Cytometry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Flow cytometric analysis of NK-92 cells labeling T-bet/TBX21. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32908, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29202416453215.jpg)
![Immunoprecipitation: T-bet/TBX21 Antibody [NBP3-14699] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MJ/G11 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiT-bet/TBX21 recombinant monoclonal antibody used for IP at 6 ul per reaction. Tbet/TBX21 was also immunoprecipitated by rabbit anti-Tbet/TBX21 antibody. For blottingimmunoprecipitated T-bet/TBX21, was used at1:1000. Chemiluminescence with an exposure time of 3seconds.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-Immunoprecipitation-NBP3-14699-img0001.jpg "Immunoprecipitation: T-bet/TBX21 Antibody [NBP3-14699] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MJ/G11 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiT-bet/TBX21 recombinant monoclonal antibody used for IP at 6 ul per reaction. Tbet/TBX21 was also immunoprecipitated by rabbit anti-Tbet/TBX21 antibody. For blottingimmunoprecipitated T-bet/TBX21, was used at1:1000. Chemiluminescence with an exposure time of 3seconds.")
![Western Blot: T-bet/TBX21 Antibody (4B10) [NBP1-43298] - CD4+ T cells were sorted from mouse spleen, activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-activated (right](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-4B10-Western-Blot-NBP1-43298-img0002.jpg "Western Blot: T-bet/TBX21 Antibody (4B10) [NBP1-43298] - CD4+ T cells were sorted from mouse spleen, activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-activated (right")
![Flow (Intracellular): T-bet/TBX21 Antibody (4B10) [NBP1-43298] - Intracellular staining of normal human peripheral blood cells with Anti-Human CD8a APC and 0.125 ug of Anti-Human/Mouse T-bet PerCP-Cy5.5 using the Foxp3 Fixation/Permeabilization Buffers . Cells in the lymphocyte gate were used for analysis. Image using the PerCP/Cy5.5 format of this antibody.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-4B10-Flow-Intracellular-NBP1-43298-img0005.jpg)
![Western Blot: T-bet/TBX21 Antibody [NBP2-41235] - Western blot analysis of TBX21 in 293 cell lysate with TBX21 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-Western-Blot-NBP2-41235-img0001.jpg "Western Blot: T-bet/TBX21 Antibody [NBP2-41235] - Western blot analysis of TBX21 in 293 cell lysate with TBX21 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: T-bet/TBX21 Antibody [NBP2-41235] - Immunofluorescence of TBX21 in 293 cells with TBX21 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41235-img0002.jpg)
![Western Blot: T-bet/TBX21 Antibody [NBP2-20583] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-Western-Blot-NBP2-20583-img0003.jpg "Western Blot: T-bet/TBX21 Antibody [NBP2-20583] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:3000.")
![Immunohistochemistry-Paraffin: T-bet/TBX21 Antibody [NBP2-20583] - DLD-1 xenograft, using TBX21 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-Immunohistochemistry-Paraffin-NBP2-20583-img0004.jpg)
![Western Blot: Tbx21 Overexpression Lysate (Adult Normal) [NBP2-07419] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tbx21.](http://images.novusbio.com/fullsize/Tbx21-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07419-img0001.jpg "Western Blot: Tbx21 Overexpression Lysate (Adult Normal) [NBP2-07419] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tbx21.")