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![Western Blot: Senataxin Antibody [NBP1-94712] - Input: HeLa whole cell lysate. Beads without antibody IP control. IP: IP from HeLa lysate.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Western-Blot-NBP1-94712-img0002.jpg "Western Blot: Senataxin Antibody [NBP1-94712] - Input: HeLa whole cell lysate. Beads without antibody IP control. IP: IP from HeLa lysate.")
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Senataxin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94712-img0010.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (transverse section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very strong staining in the cytoplasm and the nuclei of the muscle cells. No signal was found in the perimysium and endomycium area (connective tissue) of the section.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP1-94712-img0007.jpg)
![Flow Cytometry: Senataxin Antibody [NBP1-94712] - An intracellular stain was performed on Jurkat cells and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .](http://images.novusbio.com/fullsize/Senataxin-Antibody-Flow-Cytometry-NBP1-94712-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94712-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - Senataxin immunofluorescence in fibroblasts. Image from verified customer review.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94712-img0004.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (verticle section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very strong staining in the cytoplasm and the nuclei of the muscle cells. No signal was found in the perimysium and endomycium (connective tissue) of the section.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP1-94712-img0006.jpg)
![Western Blot: Senataxin Antibody [NB100-57542] - Whole cell lysate (5 ug) from HeLa, 293T, Jurkat, and A549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Western-Blot-NB100-57542-img0006.jpg "Western Blot: Senataxin Antibody [NB100-57542] - Whole cell lysate (5 ug) from HeLa, 293T, Jurkat, and A549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Senataxin Antibody [NB100-57542] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57542 used for IP at 3 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57543. For blotting immunoprecipitated Senataxin, NB100-57542 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunoprecipitation-NB100-57542-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Senataxin Antibody [NB100-57542] - SAN1 interacts with the RNA/DNA helicase sSenataxin. IB of SETX siRNA knockdown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05008-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Knockdown-Validated-NB100-57542-img0008.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody (5D8) [NBP2-52727] - Senataxin antibody clone 5D8 was tested on human prostate tissue sections and generated strong cytoplasmic-nuclear staining in the secretary/basal epithelial cells as well as the dense connective tissue.](http://images.novusbio.com/fullsize/Senataxin-Antibody-5D8-Immunohistochemistry-Paraffin-NBP2-52727-img0002.jpg "Immunohistochemistry-Paraffin: Senataxin Antibody (5D8) [NBP2-52727] - Senataxin antibody clone 5D8 was tested on human prostate tissue sections and generated strong cytoplasmic-nuclear staining in the secretary/basal epithelial cells as well as the dense connective tissue.")
![Flow Cytometry: Senataxin Antibody (5D8) [NBP2-52727] - An intracellular stain was performed on Jurkat cells with antibody NBP2-52727 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Secondary Antibody.](http://images.novusbio.com/fullsize/Senataxin-Antibody-5D8-Flow-Cytometry-NBP2-52727-img0003.jpg)
![Flow (Intracellular): Senataxin Antibody (5D8) [NBP2-52727] - An intracellular stain was performed on HepG2 cells with antibody NBP2-52727 (blue) and a matched isotype control NBP2-27231 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Senataxin-Antibody-5D8-Flow-Intracellular-NBP2-52727-img0001.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody (4G1) [NBP2-52726] - Senataxin antibody clone 4G1 was tested on human prostate tissue sections and generated strong cytoplasmic-nuclear staining in the secretary/basal epithelial cells as well as the dense connective tissue.](http://images.novusbio.com/fullsize/Senataxin-Antibody-4G1-Immunohistochemistry-Paraffin-NBP2-52726-img0002.jpg "Immunohistochemistry-Paraffin: Senataxin Antibody (4G1) [NBP2-52726] - Senataxin antibody clone 4G1 was tested on human prostate tissue sections and generated strong cytoplasmic-nuclear staining in the secretary/basal epithelial cells as well as the dense connective tissue.")
![Flow (Intracellular): Senataxin Antibody (4G1) [NBP2-52726] - An intracellular stain was performed on HepG2 cells with Senataxin Antibody (4G1) antibody NBP2-52726 (blue) and a matched isotype control NBP2-27231 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Senataxin-Antibody-4G1-Flow-Intracellular-NBP2-52726-img0001.jpg)
![Western Blot: Senataxin Antibody (2A9) [H00023064-M05] - Analysis of SETX expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Senataxin-Antibody-2A9-Western-Blot-H00023064-M05-img0006.jpg "Western Blot: Senataxin Antibody (2A9) [H00023064-M05] - Analysis of SETX expression in PC-12 (Cat # L012V1).")
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody (2A9) [H00023064-M05] - Analysis of monoclonal antibody to SETX on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Senataxin-Antibody-2A9-Immunocytochemistry-Immunofluorescence-H00023064-M05-img0004.jpg)
![Western Blot: Senataxin Antibody (2A9) [H00023064-M05] - SETX expression in Raw 264.7](http://images.novusbio.com/fullsize/Senataxin-Antibody-2A9-Western-Blot-H00023064-M05-img0001.jpg)
![Western Blot: Senataxin Antibody (2A9) [H00023064-M05] - Analysis of SETX expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Senataxin-Antibody-2A9-Western-Blot-H00023064-M05-img0007.jpg)
![ELISA: Senataxin Antibody (2A9) [H00023064-M05] - Detection limit for recombinant GST tagged SETX is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Senataxin-Antibody-2A9-ELISA-H00023064-M05-img0008.jpg)
![Western Blot: Senataxin Antibody [NB100-57543] - Detection of Human Senataxin by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Western-Blot-NB100-57543-img0002.jpg "Western Blot: Senataxin Antibody [NB100-57543] - Detection of Human Senataxin by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Senataxin Antibody [NB100-57543] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for IP at 6 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57542. For blotting immunoprecipitated Senataxin, NB100-57543 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunoprecipitation-NB100-57543-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP2-13300] - Staining of human cell line RH-30 shows localization to nucleus & cytokinetic bridge. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13300-img0004.jpg "Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP2-13300] - Staining of human cell line RH-30 shows localization to nucleus & cytokinetic bridge. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP2-13300] - Staining of human pancreas shows no positivity in exocrine glanular cells as expected.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP2-13300-img0008.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP2-13300] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP2-13300-img0005.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP2-13300] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP2-13300-img0006.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody [NBP2-13300] - Staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunohistochemistry-Paraffin-NBP2-13300-img0007.jpg)
![Western Blot: Senataxin Antibody [NBP3-14676] - Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7,Hep-G2, A-549, SW620, SK-MEL-28, Jurkat, K-562,RMPI-8226, and 786-O cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-Senataxin recombinantmonoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 30 seconds.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Western-Blot-NBP3-14676-img0001.jpg "Western Blot: Senataxin Antibody [NBP3-14676] - Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7,Hep-G2, A-549, SW620, SK-MEL-28, Jurkat, K-562,RMPI-8226, and 786-O cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-Senataxin recombinantmonoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 30 seconds.")
![Immunoprecipitation: Senataxin Antibody [NBP3-14676] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-Senataxinrecombinant monoclonal antibody used for IP at 6 ul per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibodies. For blottingimmunoprecipitated Senataxin, was used at1:1000. Chemiluminescence with an exposure time of 10seconds.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunoprecipitation-NBP3-14676-img0002.jpg)
![Flow Cytometry:Senataxin Antibody [NBP3-14676] -: Rabbit anti-Senataxinrecombinant monoclonal [BLR050F] or isotypecontrol (unshaded). Secondary: DyLight® 488-conjugatedgoat anti-rabbit IgG](http://images.novusbio.com/fullsize/nbp3-14676_rabbit-senataxin-mab-flow-cytometry-5122024111149..png)
![Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP2-58495] - Staining of human cell line A549 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58495-img0001.jpg "Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP2-58495] - Staining of human cell line A549 shows localization to nucleoplasm.")