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![Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.](http://images.novusbio.com/fullsize/HSF1-Antibody-Western-Blot-NBP1-97475-img0002.jpg "Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.")
![Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (NBP2-42206). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 ug). Lane 4: MCF7 lysate (10 ug). Lane 5: MCF7 lysate (20 ug). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42206) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Western-Blot-NBP2-42206-img0004.jpg "Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (NBP2-42206). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 ug). Lane 4: MCF7 lysate (10 ug). Lane 5: MCF7 lysate (20 ug). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42206) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Immunocytochemistry-Immunofluorescence-NBP2-42206-img0002.jpg)
![Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 . Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Western-Blot-NBP2-42206-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Immunocytochemistry-Immunofluorescence-NBP2-42206-img0003.jpg)
![Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Western-Blot-NBP2-42205-img0004.jpg "Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0003.jpg)
![Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-278202415242677.jpg "Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32444) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-29820241420917.jpg)
![Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunocytochemistry analysis of HeLa cells labeling HSF1 with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-2920241033401.jpg)
![Flow Cytometry: HSF1 Antibody (JE44-79) [NBP3-32444] - Flow cytometric analysis of HeLa cells labeling HSF1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32444, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-392024884655.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-Paraffin-NBP1-71931-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: HSF1 Antibody [NBP1-71931] - Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HSF1 Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (left), Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (center) and Cat. No. Lot1 used at a dilution](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-NBP1-71931-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody NBP1-71931 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HSF1 was also immunoprecipitated by two other rabbit anti-HSF1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunoprecipitation-NBP1-71931-img0003.jpg)
![Western Blot: HSF1 Antibody [NBP1-81665] - Analysis in human cell line PC-3.](http://images.novusbio.com/fullsize/HSF1-Antibody-Western-Blot-NBP1-81665-img0007.jpg "Western Blot: HSF1 Antibody [NBP1-81665] - Analysis in human cell line PC-3.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody [NBP1-81665] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81665-img0008.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-81665] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-Paraffin-NBP1-81665-img0005.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody (10H8) [NBP1-97504] - Analysis of human skeletal muscle tissue stained with HSF1, mAb (10H8) at 10ug/ml.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H8-Immunohistochemistry-Paraffin-NBP1-97504-img0002.jpg "Immunohistochemistry-Paraffin: HSF1 Antibody (10H8) [NBP1-97504] - Analysis of human skeletal muscle tissue stained with HSF1, mAb (10H8) at 10ug/ml.")
![Western Blot: HSF1 [p Ser 326] Antibody (SR2246) [NBP3-21520] - Western blot analysis using NBP3-21520 in HeLa cell lysates treated with heat.](http://images.novusbio.com/fullsize/nbp3-21520_rabbit-hsf1-p-ser-326-mab-sr2246-57202316112194.jpg "Western Blot: HSF1 [p Ser 326] Antibody (SR2246) [NBP3-21520] - Western blot analysis using NBP3-21520 in HeLa cell lysates treated with heat.")
![Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of Recombinant HSF1 and HSF1 (phospho): Lane 1: MWM, Lane 2: Recombinant HSF1, Lane 3: Recombinant HSF1 (phospho), probed with HSF1 (pSer326) pAb.](http://images.novusbio.com/fullsize/HSF1-[p-Ser326]-Antibody-Western-Blot-NBP1-97452-img0002.jpg "Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of Recombinant HSF1 and HSF1 (phospho): Lane 1: MWM, Lane 2: Recombinant HSF1, Lane 3: Recombinant HSF1 (phospho), probed with HSF1 (pSer326) pAb.")
![Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of HSF1 (pSer326): Lane 1: MW marker, Lane 2: HeLa, Lane 3: HeLa, Heat-Shocked (No recovery time), probed with HSF1 (pSer326) pAb.](http://images.novusbio.com/fullsize/HSF1-[p-Ser326]-Antibody-Western-Blot-NBP1-97452-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (009) [NBP2-90081] - Staining of Human HSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSF1 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunocytochemistry-Immunofluorescence-NBP2-90081-img0001.jpg "Immunocytochemistry/Immunofluorescence: HSF1 Antibody (009) [NBP2-90081] - Staining of Human HSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSF1 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red).")
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0003.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human placenta with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0002.jpg)