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![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: DIAPH-2 Antibody [NBP1-85217] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NBP1-85217-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: DIAPH-2 Antibody [NBP1-85217] - Staining of human cell line U-251 MG shows localization to nucleoli, endoplasmic reticulum and vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85217-img0016.jpg)
![Western Blot: DIAPH-2 Antibody [NBP1-85217] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NBP1-85217-img0007.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0013.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0014.jpg)
![Western Blot: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1111-Western-Blot-NBP2-52966-img0007.jpg "Western Blot: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG")
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human uterus shows strong cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1111-Immunohistochemistry-Paraffin-NBP2-52966-img0006.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human colorectal cancer shows strong cytoplasmic immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1111-Immunohistochemistry-Paraffin-NBP2-52966-img0001.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human fallopian tube shows strong cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1111-Immunohistochemistry-Paraffin-NBP2-52966-img0002.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human placenta shows strong staining in the trophoblast.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1111-Immunohistochemistry-Paraffin-NBP2-52966-img0003.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1111-Immunohistochemistry-Paraffin-NBP2-52966-img0004.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules cells.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1111-Immunohistochemistry-Paraffin-NBP2-52966-img0005.jpg)
![Western Blot: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1113-Western-Blot-NBP2-52967-img0007.jpg "Western Blot: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG")
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human uterus shows strong cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1113-Immunohistochemistry-Paraffin-NBP2-52967-img0006.jpg)
![Immunohistochemistry: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human colorectal cancer shows strong cytoplasmic immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1113-Immunohistochemistry-NBP2-52967-img0001.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human kidney shows moderate positivity in renal glomeruli.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1113-Immunohistochemistry-Paraffin-NBP2-52967-img0002.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human pancreas shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1113-Immunohistochemistry-Paraffin-NBP2-52967-img0003.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human placenta shows strong staining in the trophoblast.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1113-Immunohistochemistry-Paraffin-NBP2-52967-img0004.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1113) [NBP2-52967] - Staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules cells.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-CL1113-Immunohistochemistry-Paraffin-NBP2-52967-img0005.jpg)
![Western Blot: DIAPH-2 Antibody (V78P3C10-D3) [NBP2-50229] - Analysis of DIAPH-2 in Jurkat cell lysates.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-V78P3C10-D3-Western-Blot-NBP2-50229-img0001.jpg "Western Blot: DIAPH-2 Antibody (V78P3C10-D3) [NBP2-50229] - Analysis of DIAPH-2 in Jurkat cell lysates.")
![Immunohistochemistry: DIAPH-2 Antibody (V78P3C10-D3) [NBP2-50229] - Analysis of DIAPH-2 in human colorectal cancer tissue. Showing cytoplasmic staining.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-V78P3C10-D3-Immunohistochemistry-NBP2-50229-img0002.jpg)
![Western Blot: DIAPH-2 Antibody [NB600-280] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Diaphanous 2 antibody.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NB600-280-img0004.jpg "Western Blot: DIAPH-2 Antibody [NB600-280] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Diaphanous 2 antibody.")
![Western Blot: DIAPH-2 Antibody [NB600-280] - Whole cell lysate (50 ug) from HEK 293T cells. Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NB600-280-img0002.jpg)
![Western Blot: DIAPH-2 Antibody (V78 P3C10-D3) [NBP3-18260] - Western Blot analysis of Mouse liver showing detection of 125 kda DIAPH-2 protein using Mouse Anti-DIAPH-2 Monoclonal Antibody, Clone V78 P3C10-D3 (NBP3-18260). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver (10ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-DIAPH-2 Monoclonal Antibody (NBP3-18260) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 125 kda.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-V78-P3C10-D3-Western-Blot-NBP3-18260-img0001.jpg "Western Blot: DIAPH-2 Antibody (V78 P3C10-D3) [NBP3-18260] - Western Blot analysis of Mouse liver showing detection of 125 kda DIAPH-2 protein using Mouse Anti-DIAPH-2 Monoclonal Antibody, Clone V78 P3C10-D3 (NBP3-18260). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver (10ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-DIAPH-2 Monoclonal Antibody (NBP3-18260) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 125 kda.")
![Western Blot: DIAPH-2 Antibody [NBP1-98364] - Titration: 1.0 ug/ml Positive Control: Rat Brain.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NBP1-98364-img0002.jpg "Western Blot: DIAPH-2 Antibody [NBP1-98364] - Titration: 1.0 ug/ml Positive Control: Rat Brain.")