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![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Analysis in human kidney and pancreas tissues. Corresponding DBT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Analysis in human kidney and pancreas tissues. Corresponding DBT RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon, liver, lymph node and pancreas using Anti-DBT antibody NBP1-89522 (A) shows similar protein distribution across tissues to independent antibody NBP1-85964 (B).](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0013.jpg)
![Western Blot: DBT Antibody [NBP1-89522] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-89522-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89522-img0007.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0015.jpg)
![Western Blot: DBT Antibody [NBP1-89522] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-89522-img0005.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0008.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human testis shows weak to moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0009.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0010.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0011.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human liver.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0014.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human lymph node.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0016.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85963] - Staining in human kidney and pancreas tissues using anti-DBT antibody. Corresponding DBT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85963-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85963] - Staining in human kidney and pancreas tissues using anti-DBT antibody. Corresponding DBT RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: DBT Antibody [NBP1-85963] - Analysis in control (vector only transfected HEK293T lysate) and DBT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-85963-img0008.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85963] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85963-img0014.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85963] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85963-img0005.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85963] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85963-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85963] - Staining of human colon, liver, lymph node and pancreas using Anti-DBT antibody NBP1-85963 (A) shows similar protein distribution across tissues to independent antibody NBP1-85964 (B).](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-85963-img0009.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85963] - Staining of human liver.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85963-img0010.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85963] - Staining of human lymph node.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85963-img0011.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85963] - Staining of human colon.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85963-img0012.jpg)
![Western Blot: DBT Antibody [NBP1-85964] - Staining of human colon, liver, lymph node and pancreas using Anti-DBT antibody NBP1-85964 (A) shows similar protein distribution across tissues to independent antibody NBP1-89522 (B).](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-85964-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DBT Antibody [NBP1-85964] - Staining of human colon, liver, lymph node and pancreas using Anti-DBT antibody NBP1-85964 (A) shows similar protein distribution across tissues to independent antibody NBP1-89522 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85964] - Staining of human colon.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85964-img0010.jpg)
![Western Blot: DBT Antibody [NBP1-85964] - Analysis in control (vector only transfected HEK293T lysate) and DBT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-85964-img0007.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85964] - Staining of human kidney shows strong cytoplasmic positivity in distal tubules.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85964-img0002.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85964] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85964-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85964] - Staining in human kidney and pancreas tissues using anti-DBT antibody. Corresponding DBT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85964-img0006.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85964] - Staining of human liver.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85964-img0009.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-85964] - Staining of human lymph node.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-85964-img0011.jpg)
![Western Blot: DBT Antibody [NBP1-31760] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DBT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-31760-img0011.jpg "Western Blot: DBT Antibody [NBP1-31760] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DBT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-31760] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DBT stained by DBT antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31760-img0009.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-31760] - Mouse kidney. DBT antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-31760-img0004.jpg)
![Western Blot: DBT Antibody [NBP1-31760] - Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-31760-img0003.jpg)
![Western Blot: DBT Antibody [NBP1-31760] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE DBT antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-31760-img0005.jpg)
![Western Blot: DBT Antibody [NBP1-31760] - A. 50 ug mouse liver lysate/extract 10% SDS-PAGE DBT antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP1-31760-img0006.jpg)
![Western Blot: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.](http://images.novusbio.com/fullsize/DBT-Antibody-OTI1G2-Western-Blot-NBP2-02042-img0002.jpg "Western Blot: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.")
![Immunocytochemistry/Immunofluorescence: DBT Antibody (OTI1G2) [NBP2-02042] - Anti-DBT mouse monoclonal antibody (NBP2-02042) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT.](http://images.novusbio.com/fullsize/DBT-Antibody-OTI1G2-Immunocytochemistry-Immunofluorescence-NBP2-02042-img0003.jpg)
![Flow Cytometry: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DBT antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/DBT-Antibody-OTI1G2-Flow-Cytometry-NBP2-02042-img0001.jpg)
![Western Blot: DBT Antibody [NBP3-16010] - Western blot analysis of extracts of various cell lines, using DBT antibody (NBP3-16010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/DBT-Antibody-Western-Blot-NBP3-16010-img0002.jpg "Western Blot: DBT Antibody [NBP3-16010] - Western blot analysis of extracts of various cell lines, using DBT antibody (NBP3-16010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: DBT Antibody [NBP3-16010] - Immunohistochemistry of paraffin-embedded mouse kidney using DBT Rabbit pAb (NBP3-16010) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DBT-Antibody-Immunohistochemistry-Paraffin-NBP3-16010-img0001.jpg)
![Western Blot: DBT Overexpression Lysate (Adult Normal) [NBL1-09735] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DBT.](http://images.novusbio.com/fullsize/DBT-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09735-img0002.jpg "Western Blot: DBT Overexpression Lysate (Adult Normal) [NBL1-09735] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DBT.")