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![N/A Complement Component C5a [Biotin]](http://images.novusbio.com/fullsize2/DATA_Complement_Component_C5a_DY2037_ELISA_1753.jpg "N/A Complement Component C5a [Biotin]")
![Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-278202415295515.jpg "Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32209) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29820241435282.jpg)
![Immunocytochemistry/ Immunofluorescence: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunocytochemistry analysis of HepG2 cells labeling Complement C5 with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-2920241192373.jpg)
![Flow Cytometry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Flow cytometric analysis of HepG2 cells labeling Complement C5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32209, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29202416372127.jpg)
![Western Blot: Complement Component C5a Antibody (D4) [NBP3-41581] - Sample: Rat Serum Primary Ab: 3ug/ml NBP3-41581; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41581_mouse-complement-component-c5a-mab-d4-61220242124321.jpg "Western Blot: Complement Component C5a Antibody (D4) [NBP3-41581] - Sample: Rat Serum Primary Ab: 3ug/ml NBP3-41581; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Complement Component C5a Antibody (D4) [NBP3-41581] - Sample: Lane1: Human Serum; Lane2: Human Plasma Primary Ab: 5ug/ml NBP3-41581; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41581_mouse-complement-component-c5a-mab-d4-612202421373023.jpg)
![Immunohistochemistry-Paraffin: Complement Component C5a Antibody (D4) [NBP3-41581] - DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 30ug/ml NBP3-41581; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41581_mouse-complement-component-c5a-mab-d4-61220242058817.jpg)
![Immunohistochemistry-Paraffin: Complement Component C5a Antibody (D5) [NBP3-41582] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41582; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41582_mouse-complement-component-c5a-mab-d5-612202421283828.jpg "Immunohistochemistry-Paraffin: Complement Component C5a Antibody (D5) [NBP3-41582] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41582; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Complement Component C5a Antibody (D5) [NBP3-41582] - DAB staining on IHC-P; Sample: Human Spleen Tissue; Primary Ab: 30ug/ml NBP3-41582; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41582_mouse-complement-component-c5a-mab-d5-612202421331652.jpg)
![Immunohistochemistry-Paraffin: Complement Component C5a Antibody (D5) [NBP3-41582] - DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 30ug/ml NBP3-41582; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41582_mouse-complement-component-c5a-mab-d5-61220242139536.jpg)
![Immunohistochemistry-Paraffin: Complement C5 Antibody [NBP1-84395] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Complement-C5-Antibody-Immunohistochemistry-Paraffin-NBP1-84395-img0007.jpg "Immunohistochemistry-Paraffin: Complement C5 Antibody [NBP1-84395] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.")
![Immunohistochemistry-Paraffin: Complement C5 Antibody [NBP1-84395] - Staining of human small intestine shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Complement-C5-Antibody-Immunohistochemistry-Paraffin-NBP1-84395-img0005.jpg)
![Immunohistochemistry-Paraffin: Complement C5 Antibody [NBP1-84395] - Staining of human lymph node shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Complement-C5-Antibody-Immunohistochemistry-Paraffin-NBP1-84395-img0006.jpg)
![Immunohistochemistry-Paraffin: Complement C5 Antibody [NBP1-84395] - Staining of human placenta shows moderate positivity in plasma.](http://images.novusbio.com/fullsize/Complement-C5-Antibody-Immunohistochemistry-Paraffin-NBP1-84395-img0008.jpg)
![Western Blot: Complement Component C5a Antibody (D2) [NBP3-41586] - Sample: Rat Serum Primary Ab: 3ug/ml NBP3-41586; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41586_mouse-complement-component-c5a-mab-d2-612202421351220.jpg "Western Blot: Complement Component C5a Antibody (D2) [NBP3-41586] - Sample: Rat Serum Primary Ab: 3ug/ml NBP3-41586; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Complement Component C5a Antibody (D2) [NBP3-41586] - Sample: Rat Serum Primary Ab: 3ug/ml NBP3-41586; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41586_mouse-complement-component-c5a-mab-d2-612202421175935.jpg)