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Complement Component C5/C5a Antibody (295003) [Unconjugated]

 (9 Publications)
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Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N‑acetyl‑ beta ‑D‑glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosamin­idase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concen­trations of Mouse Anti-Human Complement Component C5a Monoclonal Antibody (Catalog # MAB2037). The ND50 is typically 0.04‑0.12 µg/mL in the presence of cytochalasin‑B.
Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N‑acetyl‑ beta ‑D‑glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
295003
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2037-SP
Carrier Free
MAB2037-500
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Complement Component C5/C5a Antibody (295003) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Complement Component C5a
Thr678-Arg751
Accession # P01031
Specificity
Detects human Complement Component C5/C5a in ELISAs and Western blots. In sandwich immunoassays, detects human Complement Component C5a by itself or in the context of Complement Component C5. In Western blots, this antibody does not cross-react with recombinant human Complement Component C2, recombinant mouse (rm) Complement Component C3d, or rmComplement Component C5a.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
C5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.04-0.12 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human Complement Component C5/C5a Antibody (Catalog # MAB2037)
ELISA Detection: Human Complement Component C5/C5a Biotinylated Antibody (Catalog # BAM20371)
Standard: Recombinant Human Complement Component C5a (Catalog # 2037-C5)
Publications
Read Publications using
MAB2037 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C5/C5a Antibody (295003) [Unconjugated]

  • C5
  • C5a;C5b;C5D;Complement C5;CPAMD4;ECLZB
  • Complement Component C5/C5a

Background

Human complement component C5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a non-signaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

  1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
  5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Complement Component C5/C5a Antibody (MAB2037)(9)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 4 applications: Bioassay, ELISA Capture, ELISA Development, ELISA Development (Capture).


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Bioassay
(1)
ELISA Capture
(3)
ELISA Development
(1)
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Human
(6)
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(1)
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Showing Publications 1 - 9 of 9.
Publications using MAB2037 Applications Species
Ullgren, A;Öijerstedt, L;Olofsson, J;Bergström, S;Remnestål, J;van Swieten, JC;Jiskoot, LC;Seelaar, H;Borroni, B;Sanchez-Valle, R;Moreno, F;Laforce, R;Synofzik, M;Galimberti, D;Rowe, JB;Masellis, M;Tartaglia, MC;Finger, E;Vandenberghe, R;de Mendonça, A;Tirabosch, P;Santana, I;Ducharme, S;Butler, CR;Gerhard, A;Otto, M;Bouzigues, A;Russell, L;Swift, IJ;Sogorb-Esteve, A;Heller, C;Rohrer, JD;Månberg, A;Nilsson, P;Graff, C;Genetic Frontotemporal Dementia Initiative (GENFI), ; Altered plasma protein profiles in genetic FTD - a GENFI study Molecular neurodegeneration 2023-11-15 [PMID: 37968725] (Bioassay, Human) Bioassay Human
Chen Li, Tianli Chen, Jialiang Liu, Yue Wang, Chunhuan Zhang, Lu Guo et al. FGF19‐Induced Inflammatory CAF Promoted Neutrophil Extracellular Trap Formation in the Liver Metastasis of Colorectal Cancer Advanced Science 8/1/2023 [PMID: 37345586]
CR Toy, H Song, HN Nagaraja, J Scott, J Greco, X Zhang, CY Yu, JA Tumlin, BH Rovin, LA Hebert, DJ Birmingham The Influence of an Elastase-Sensitive Complement C5 Variant on Lupus Nephritis and Its Flare Kidney international reports, 2021-06-09;6(8):2105-2113. 2021-06-09 [PMID: 34386659] (ELISA Capture, Human) ELISA Capture Human
TA Shah, HK Pallera, CL Kaszowski, WT Bass, FA Lattanzio Therapeutic Hypothermia Inhibits the Classical Complement Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy Frontiers in Neuroscience, 2021-02-12;15(0):616734. 2021-02-12 [PMID: 33642979] (ELISA Capture, Rat) ELISA Capture Rat
I Ayoub, JP Shapiro, H Song, XL Zhang, S Parikh, S Almaani, S Madhavan, SV Brodsky, A Satoskar, C Bott, L Yu, M Merchant, J Klein, JM Mejia-Vile, T Nadasdy, D Birmingham, BH Rovin Establishing a Case for Anti-complement Therapy in Membranous Nephropathy Kidney international reports, 2020-12-13;6(2):484-492. 2020-12-13 [PMID: 33615073] (ELISA Capture, Human) ELISA Capture Human
Sonika Rathi, Subhadra Jalali, Satish Patnaik, Shahna Shahulhameed, Ganeswara R. Musada, Divya Balakrishnan et al. Abnormal Complement Activation and Inflammation in the Pathogenesis of Retinopathy of Prematurity Frontiers in Immunology 12/22/2017 [PMID: 29312345]
Georgoutsou-Spyridonos M, Ricklin D, Pratsinis H, Perivolioti E, Pirmettis I, Garcia B, Geisbrecht B, Foukas P, Lambris J, Mastellos D, Sfyroera G Attenuation of Staphylococcus aureus-Induced Bacteremia by Human Mini-Antibodies Targeting the Complement Inhibitory Protein Efb. J Immunol, 2015-09-04;195(8):3946-58. 2015-09-04 [PMID: 26342032] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Ramadass , Mahalaks, Ghebrehiwet , Berhane, Smith , Richard, Kew , Richard Generation of multiple fluid-phase C3b:plasma protein complexes during complement activation: possible implications in C3 glomerulopathies. J Immunol, 2013-12-23;192(3):1220-30. 2013-12-23 [PMID: 24367026] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Kambas K, Markiewski MM, Pneumatikos IA, Rafail SS, Theodorou V, Konstantonis D, Kourtzelis I, Doumas MN, Magotti P, Deangelis RA, Lambris JD, Ritis KD C5a and TNF-alpha up-regulate the expression of tissue factor in intra-alveolar neutrophils of patients with the acute respiratory distress syndrome. J. Immunol., 2008-06-01;180(11):7368-75. 2008-06-01 [PMID: 18490736] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol C5
Uniprot