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![Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0007.jpg "Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Immunocytochemistry-Immunofluorescence-NB100-308-img0001.jpg)
![Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0005.jpg)
![Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0004.jpg "Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ATR Antibody [NB100-323] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATR antibody NB100-323 lot 3 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ATR was also immunoprecipitated by a previous lot (lot 2) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0002.jpg)
![Western Blot: ATR Antibody [NB100-323] - H1299 (human lung cancer cells) and HCC 44 (human liver cancer cells). Antibody at 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0003.jpg)
![Western Blot: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Western-Blot-NBP3-13665-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Immunocytochemistry-Immunofluorescence-NBP3-13665-img0001.jpg)
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Immunohistochemistry-Paraffin-NBP3-13665-img0004.jpg)
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Immunohistochemistry-Paraffin-NBP3-13665-img0002.jpg)
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Rat lung.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13665_rabbit-atr-p-thr-1989-mab-hl132-immunohistochemistry-paraffin-910202391111..jpg)
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Mouse tissue.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13665_rabbit-atr-p-thr-1989-mab-hl132-immunohistochemistry-paraffin-910202391240..jpg)
![Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05447-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Rat testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0005.jpg)
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Human testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0003.jpg)
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Mouse testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0004.jpg)
![Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of 293T, using Phospho-ATR-S428 antibody at 1:400 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05447_rabbit-polyclonal-atr-p-ser428-antibody-western-blot-265202385445.jpg)
![Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of NIH/3T3, using Phospho-ATR-S428 antibody at 1:400 dilution.NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05447_rabbit-polyclonal-atr-p-ser428-antibody-western-blot-265202385627.jpg)
![Immunohistochemistry-Paraffin: ATR Antibody (1E9) [H00000545-M03] - Analysis of monoclonal antibody to ATR on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/ATR-Antibody-1E9-Immunohistochemistry-Paraffin-H00000545-M03-img0002.jpg "Immunohistochemistry-Paraffin: ATR Antibody (1E9) [H00000545-M03] - Analysis of monoclonal antibody to ATR on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml")
![Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [222]. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-222-Western-Blot-NBP2-43564-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [222]. The signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-222-Immunoprecipitation-NBP2-43564-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-910202419593539.jpg)
![Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [GT222] (NBP2-43564) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242014673.jpg)
![Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242075915.jpg)
![Immunocytochemistry/Immunofluorescence: ATR Antibody [NBP2-98982] - Immunofluorescence staining of ATR in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ATR polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/ATR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98982-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATR Antibody [NBP2-98982] - Immunofluorescence staining of ATR in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ATR polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .")
![Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in mouse testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunohistochemistry-Paraffin-NBP2-98982-img0004.jpg)
![Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in human prostate with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunohistochemistry-Paraffin-NBP2-98982-img0002.jpg)
![Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in human testis with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunohistochemistry-Paraffin-NBP2-98982-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATR Antibody (3F2) [H00000545-M01] - Analysis of monoclonal antibody to ATR on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ATR-Antibody-3F2-Immunocytochemistry-Immunofluorescence-H00000545-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: ATR Antibody (3F2) [H00000545-M01] - Analysis of monoclonal antibody to ATR on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: ATR Antibody (3F2) [H00000545-M01] - Detection limit for recombinant GST tagged ATR is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATR-Antibody-3F2-ELISA-H00000545-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATR Antibody [NBP2-76547] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76547-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATR Antibody [NBP2-76547] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.")