![Western Blot: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Western-Blot-NBP3-13665-img0003.jpg "Western Blot: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL132 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide surrounding phospho Thr1989 of human ATR. The exact sequence is proprietary. |
| Modification | p Thr1989 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ATR |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 301 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for ATR Antibody (NBP3-13665)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ATR |
![Immunocytochemistry/Immunofluorescence: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Immunocytochemistry-Immunofluorescence-NBP3-13665-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Immunohistochemistry-Paraffin-NBP3-13665-img0004.jpg "Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Immunohistochemistry-Paraffin-NBP3-13665-img0002.jpg "Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Rat lung.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13665_rabbit-atr-p-thr-1989-mab-hl132-immunohistochemistry-paraffin-910202391111..jpg "Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Rat lung.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Mouse tissue.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13665_rabbit-atr-p-thr-1989-mab-hl132-immunohistochemistry-paraffin-910202391240..jpg "Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Mouse tissue.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")