| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1268-SP | |||
| Carrier Free | MAB1268-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90309-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1268-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52557-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74095 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90308-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NB120-7635-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2979-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB12681-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF12681-SP | |||
![N/A Serpin A1/alpha 1-Antitrypsin [Biotin]](http://images.novusbio.com/fullsize2/DATA_Serpin_A1_DY1268_ELISA_1616.jpg "N/A Serpin A1/alpha 1-Antitrypsin [Biotin]")
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Analysis in human kidney and cerebral cortex tissues using NBP1-90309 antibody. Corresponding SERPINA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90309-img0025.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Analysis in human kidney and cerebral cortex tissues using NBP1-90309 antibody. Corresponding SERPINA1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human cerebral cortex, kidney, placenta and testis using Anti-SERPINA1 antibody NBP1-90309 (A) shows similar protein distribution across tissues to independent antibody NBP1-90308 (B).](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90309-img0024.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Analysis using Anti-SERPINA1 antibody NBP1-90309 (A) shows similar pattern to independent antibody NBP1-90308 (B).](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NBP1-90309-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90309-img0009.jpg)
![Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Assessment of CCL18 and A1AT (Serpin A1/alpha 1-Antitrypsin) in bladder tissue. Representative immunostaining of benign bladder for A1AT (top-left), low-grade non-muscle invasive BCa for A1AT (top-middle), and high-grade muscle invasive BCa for A1AT (top-right). A1AT staining was present in the cytoplasm and stroma. A1AT staining varied from weak and focal to strong and diffuse. Lower panel shows column bar graphs of A1AT immunohistochemical staining intensity of benign bladder vs. bladder cancer, low-grade vs. high-grade, NMIBC vs. MIBC. Image collected and cropped by CiteAb from the following publication (//bmcurol.biomedcentral.com/articles/10.1186/1471-2490-13-42), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-NBP1-90309-img0030.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Analysis in human plasma.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NBP1-90309-img0021.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human kidney shows moderate positivity in plasma.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90309-img0027.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human placenta shows strong positivity in plasma in blood vessels.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90309-img0028.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90309-img0029.jpg)
![Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Representative expression status for SERPINA1 level in benign tissue. All images were captured at 400x magnification. Image collected and cropped by CiteAb from the following publication (//diagnosticpathology.biomedcentral.com/articles/10.1186/s13000-014-0200-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-NBP1-90309-img0031.jpg)
![Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Representative expression status for ANG, CA9, MMP9, MMP10, SERPINA1, APOE, SDC1, VEGFA, serpine1 & IL8 levels in tumor tissue.Insert Representative expression status for ANG, CA9, MMP9, MMP10, SERPINA1, APOE, SDC1, VEGFA, serpine1 & IL-8 levels in benign tissue. All images were captured at 400× magnification. Image collected & cropped by CiteAb from the following publication (//diagnosticpathology.biomedcentral.com/articles/10.1186/s13000-014-0200-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90309_rabbit-polyclonal-serpin-a1-alpha-1-antitrypsin-antibody-310202415304294.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - SerpinA1 was regulated by SnailA. DLD-1 & SW480 cells were transfected with pcDNA-Snail (Snail), control vector pcDNA (vector), Snail siRNA (siSnail), or nontargeting siRNA (siNT), & Snail & serpinA1 protein levels were evaluated by western blot analysis. B. DLD-1 & SW480 cells were transfected with pcDNA-serpinA1 (serpinA1), control vector pcDNA (vector), serpinA1 siRNA (siSerpinA1), or nontargeting siRNA (siNT), & western blot analysis was performed for detection of Snail & SerpinA1 expression. C. DLD-1 & SW480 cells were transfected with pcDNA-Snail (Snail) or control vector pcDNA (vector), & ChIP assays were performed. The presence of the serpinA1 promoter (−516/−4) was verified in immunoprecipitates with either mouse IgG or anti-Snail antibodies, & assay inputs were analyzed using real-time PCR. The samples were loaded on agarose gels. D. Data show promoter enrichment in the anti-Snail immunoprecipitate relative to IgG. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26015410), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90309_rabbit-polyclonal-serpin-a1-alpha-1-antitrypsin-antibody-310202416121991.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - SerpinA1 was regulated by SnailA. DLD-1 & SW480 cells were transfected with pcDNA-Snail (Snail), control vector pcDNA (vector), Snail siRNA (siSnail), or nontargeting siRNA (siNT), & Snail & serpinA1 protein levels were evaluated by western blot analysis. B. DLD-1 & SW480 cells were transfected with pcDNA-serpinA1 (serpinA1), control vector pcDNA (vector), serpinA1 siRNA (siSerpinA1), or nontargeting siRNA (siNT), & western blot analysis was performed for detection of Snail & SerpinA1 expression. C. DLD-1 & SW480 cells were transfected with pcDNA-Snail (Snail) or control vector pcDNA (vector), & ChIP assays were performed. The presence of the serpinA1 promoter (−516/−4) was verified in immunoprecipitates with either mouse IgG or anti-Snail antibodies, & assay inputs were analyzed using real-time PCR. The samples were loaded on agarose gels. D. Data show promoter enrichment in the anti-Snail immunoprecipitate relative to IgG. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26015410), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90309_rabbit-polyclonal-serpin-a1-alpha-1-antitrypsin-antibody-31020241613640.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis using SERPINA1 mAb against HEK293 (1) and SERPINA1 (AA: 23-237)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Western-Blot-NBP2-52557-img0012.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis using SERPINA1 mAb against HEK293 (1) and SERPINA1 (AA: 23-237)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Immunofluorescence analysis of GC-7901 cells using SERPINA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Immunofluorescence-NBP2-52557-img0010.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of paraffin-embedded bladder cancer tissues using SERPINA1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Immunohistochemistry-Paraffin-NBP2-52557-img0009.jpg)
![Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - An intracellular stain was performed on A549 cells with Serpin AI/alpha I-Antitrypsin antibody (5DID2) NBP2-52557APC (blue) and a matched isotype control NBP1-97005APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Flow-Cytometry-NBP2-52557-img0013.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis using SERPINA1 mAb against human SERPINA1 (AA: 23-237) recombinant protein. (Expected MW is 50.3 kDa)](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Western-Blot-NBP2-52557-img0003.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis using SERPINA1 mAb against HEK293 (1) and SERPINA1 (AA: 23-237)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Western-Blot-NBP2-52557-img0004.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis using SERPINA1 mAb against human SERPINA1 (AA: 23-237) recombinant protein. (Expected MW is 50.3 kDa)](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Western-Blot-NBP2-52557-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of GC-7901 cells using SERPINA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Immunocytochemistry-Immunofluorescence-NBP2-52557-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of HepG2 cells using SERPINA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Immunocytochemistry-Immunofluorescence-NBP2-52557-img0006.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of paraffin-embedded ovarian cancer tissues using SERPINA1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Immunohistochemistry-Paraffin-NBP2-52557-img0008.jpg)
![Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of Hela cells using SERPINA1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-Flow-Cytometry-NBP2-52557-img0007.jpg)
![ELISA: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Black line: Control Antigen (100 ng); Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-5D1D2-ELISA-NBP2-52557-img0002.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Western blot analysis of extracts (10ug) from 7 Human tissue by using NBP1-47957 ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Western-Blot-NBP1-47957-img0021.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Western blot analysis of extracts (10ug) from 7 Human tissue by using NBP1-47957 ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using NBP1-47957. (Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10min)](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP1-47957-img0015.jpg)
![Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-alpha 1 Antitrypsin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Flow-Cytometry-NBP1-47957-img0010.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Western blot analysis of extracts (35ug) from 9 different cell lines by using NBP1-47957.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Western-Blot-NBP1-47957-img0019.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with NBP1-47957.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Western-Blot-NBP1-47957-img0020.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using NBP1-47957. (Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10min)](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP1-47957-img0011.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using NBP1-47957. (Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10min)](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP1-47957-img0012.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using NBP1-47957. (Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10min)](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP1-47957-img0013.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using NBP1-47957. (Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10min)](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP1-47957-img0014.jpg)
![Immunoprecipitation: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) [NBP1-47957] - Immunoprecipitation (IP) of SERPINA1 by using TrueMab monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SERPINA1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-OTI9A1-Immunoprecipitation-NBP1-47957-img0018.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Analysis using Anti-SERPINA1 antibody NBP1-90308 (A) shows similar pattern to independent antibody NBP1-90309 (B).](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NBP1-90308-img0020.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Analysis using Anti-SERPINA1 antibody NBP1-90308 (A) shows similar pattern to independent antibody NBP1-90309 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90308-img0005.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human placenta shows strong positivity in plasma in blood vessels.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0025.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NBP1-90308-img0006.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0007.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0008.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human testis using Anti-SERPINA1 antibody.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0011.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human liver using Anti-SERPINA1 antibody.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0012.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human lung using Anti-SERPINA1 antibody.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0013.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human lymph node using Anti-SERPINA1 antibody.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human cerebral cortex, kidney, placenta and testis using Anti-SERPINA1 antibody NBP1-90308 (A) shows similar protein distribution across tissues to independent antibody NBP1-90309 (B).](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0021.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Analysis in human kidney and cerebral cortex tissues using NBP1-90308 antibody. Corresponding SERPINA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0022.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human testis shows moderate positivity in plasma.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0023.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human kidney shows moderate positivity in plasma.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0024.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90308-img0026.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0014.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.")
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Albumin depleted human serum. Lane 2: none. Load: 10 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0010.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: incubated with blocking buffer for alpha-1 Anti-Trypsin. Other band(s): none](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0011.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: at 30 min RT](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0012.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4C](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0013.jpg)
