| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5999-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34613-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52436-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08789 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2615-0.025mg | |||
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p < 0.05, ** p < 0.01, and *** p < 0.001. (c) The blots show the protein expression levels in MRC-5 and HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26339636) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p < 0.05, ** p < 0.01, and *** p < 0.001. (c) The blots show the protein expression levels in MRC-5 and HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26339636) licensed under a CC-BY license.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody [NBP1-31330] - Detects Cyclin A2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31330-img0020.jpg)
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - 293T cells stained with Cyclin A2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunofluorescence-NBP1-31330-img0010.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug Neuro2A whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0006.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0007.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0013.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Cyclin A2 Antibody [NBP1-31330] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0014.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0016.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0019.jpg)
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - NCIN87 Xenograft, using cyclin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunohistochemistry-Paraffin-NBP1-31330-img0004.jpg)
![Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of Cyclin A2 protein by 2. 5 ug of Cyclin A2 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunoprecipitation-NBP1-31330-img0009.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young & old MRC-5 & HFF fibroblasts measured by RNA-seq, qRT-PCR, & Western Blots. (a) Four genes commonly downregulated & (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first & the last PD investigated here (RNA-seq: log 2 RPKM FC; qRT-PCR: log 2− delta delta CT; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young & old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: ∗p < 0.05, ∗∗p < 0.01, & ∗∗∗p < 0.001. (c) The blots show the protein expression levels in MRC-5 & HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26339636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-3102024161600.jpg)
![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0007.jpg "Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of U-2 OS cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunocytochemistry-Immunofluorescence-NBP3-15625-img0006.jpg)
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded mouse liver using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunohistochemistry-Paraffin-NBP3-15625-img0003.jpg)
![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of Mouse testis, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of C6 cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunocytochemistry-Immunofluorescence-NBP3-15625-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunofluorescence analysis of NIH-3T3 cells using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunocytochemistry-Immunofluorescence-NBP3-15625-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded rat spleen using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunohistochemistry-Paraffin-NBP3-15625-img0001.jpg)
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin A2 Rabbit mAb (NBP3-15625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Immunohistochemistry-Paraffin-NBP3-15625-img0002.jpg)
![Western Blot: Cyclin A2 Antibody (E67) [NBP2-34311] - Western Blot Analysis of HeLa cell lysate using Cyclin A2 Antibody (E67).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-E67-Western-Blot-NBP2-34311-img0003.jpg "Western Blot: Cyclin A2 Antibody (E67) [NBP2-34311] - Western Blot Analysis of HeLa cell lysate using Cyclin A2 Antibody (E67).")
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (E67) [NBP2-34311] - Formalin-fixed, paraffin-embedded human Endometrium stained with Cyclin A2 Antibody (E67).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-E67-Immunohistochemistry-Paraffin-NBP2-34311-img0002.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0007.jpg "Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.")
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0003.jpg)
![Flow Cytometry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - 4: Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Flow-Cytometry-NBP2-52436-img0001.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA: 105-233)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0005.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against human CCNA2 (AA: 105-233) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0004.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0002.jpg)
![ELISA: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-ELISA-NBP2-52436-img0006.jpg)
![Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60kDa for Cyclin A.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NB600-495-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60kDa for Cyclin A.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NB600-495] - Anti-Cyclin A antibody was diluted 1:500 to detect Cyclin A in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunohistochemistry-Paraffin-NB600-495-img0002.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-88156] - Analysis in human cell lines A-431 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-88156-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Cyclin A2 Antibody [NBP1-88156] - Analysis in human cell lines A-431 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody [NBP1-88156] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88156-img0005.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-88156] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-88156-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) [NBP3-07468] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cyclin A2 Mouse Monoclonal Antibody (CCNA2/2333).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-CCNA2-2333-Immunohistochemistry-Paraffin-NBP3-07468-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) [NBP3-07468] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cyclin A2 Mouse Monoclonal Antibody (CCNA2/2333).")
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) [NBP3-07468] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cyclin A2 Mouse Monoclonal Antibody (CCNA2/2333).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-CCNA2-2333-Immunohistochemistry-Paraffin-NBP3-07468-img0001.jpg)
![Western Blot: Cyclin A2 Overexpression Lysate (Adult Normal) [NBL1-08864] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclin A2.](http://images.novusbio.com/fullsize/Cyclin-A2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08864-img0002.jpg "Western Blot: Cyclin A2 Overexpression Lysate (Adult Normal) [NBL1-08864] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclin A2.")
![SDS-Page: Recombinant Human Cyclin A2 Protein [NBP1-98953] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Cyclin-A2-Protein-SDS-Page-NBP1-98953-img0001.jpg "SDS-Page: Recombinant Human Cyclin A2 Protein [NBP1-98953] - 15% SDS-PAGE (3ug)")