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![Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, NBP2-50028, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. NBP2-50028 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-50028-img0004.jpg "Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, NBP2-50028, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. NBP2-50028 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Cultured mouse embryonic kidney stained with Calbindin antibody (1:200) and DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50028-img0005.jpg)
![Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028] - Representative images of horizontal cells (Calbindin D-28K, cyan) in wild type control and C1q-/- mice at P8, P13, P17, and P23. Horizontal cells in C1q-/- mice fail to remain confined to the OPL, showing an increased number of ectopic neurites (arrowheads) that peak at P13 but remain present as development proceeds. These neurites extend into the outer nuclear layer and grow longer over time. Scale bars = 25 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33177995/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-NBP2-50028-img0007.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat brain lysate (left), 0.5 ug of His-tagged recombinant proteins (right) were probed with NBP2-50028 at 1:5,000. Lane 1: Parvalbumin, Lane 2: Calretinin, Lane 3: Calbindin.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-50028-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Adult rat brain cortex (Left) and striatum (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained with NBP2-50028 (1:1,000, red), and monoclonal mouse anti-RBFOX3/NeuN (NBP1-92693; green). Calbindin labels a subset of sparsely-distributed interneurons (calbindin-positive interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Right). Since neurons also express RBFOX3/NeuN, calbindin-positive cells appear to be gold to yellow. Insets are high magnification images of boxed area of each image. Blue is Dapi nucleus staining.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50028-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat cerebellum section stained with chicken Calbindin D-28K pAb, dilution 1:2,000 (Green), and costained with rabbit MeCP2 pAb, dilution 1:5,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. Calbindin D-28K, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Free-Floating-NBP2-50028-img0006.jpg)
![Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028] - C1q is expressed in retina microglia during development. (A) qRT-PCR for C1q at P9, P14, & 14 weeks. Values represent the fold mRNA expression level relative to the levels detected in adult animals following normalization to GAPDH. (B) Representative fluorescent in situ hybridization images at P13 of C1q (green) co-stained with either Iba1 (magenta) or calbindin (red) to label microglia & horizontal cells, respectively. C1q colocalizes with microglia but not with horizontal cells in the outer retina at this time (arrowheads). Inset shows C1q expression in Iba1-positive microglia that is absent in calbindin-positive horizontal cells. Scale bars = 25 & 10 μm (inset). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33177995), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50028_chicken-polyclonal-calbindin-d-28k-antibody-310202415175235.jpg)
![Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Myh14 localizes to apical membranes of Atp6v1b1‐positive intercalated cells in the distal tubule. Adult kidney sections were stained for calbindin‐D28K, Atp6v1b1, Aqp2, & Myh14 to identify intercalated cells within the distal & connecting tubule segments as well as to confirm Myh14 localization to the intercalated cell type. Cells with positive staining for Atp6v1b1 (red) can be found in tubule segments positive for calbindin‐D28K only (A–D) as well as in tubules positive for both calbindin‐D28K & Aqp2 (E–H); in both cases Atp6v1b1 staining is observed along the basolateral membrane indicating beta ‐ intercalated cell type (arrowhead, D&H). We also observed Atp6v1b1 staining in majority of the tubules along the apical membrane indicating the alpha ‐ intercalated cell type (arrowhead, I–L). Myh14 localized to the apical membrane of the intercalated cell type in the distal tubule (arrowhead, M–P). Sections were mounted with vectashield containing DAPI to stain the nucleus (blue; D, H, L & P). Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29208685), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50028_chicken-polyclonal-calbindin-d-28k-antibody-310202416121944.jpg)
![Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028] - Horizontal cells develop normally with the loss of either C3aR or CR3. (A,B) qRT-PCR for complement proteins C3aR & CR3 at P6, P13, & adult in wild type mice. Values represent the fold mRNA expression level relative to the levels detected in control animals following normalization to GAPDH. N = 3 animals. Representative images of horizontal cells (C, anti-calbindin, cyan) & quantifications (D,E) of ectopic horizontal cell neurites in control & C3aR–/– or CR3–/– mice at P13. Horizontal cell neurites in C3aR–/– & CR3–/– mice remain confined to the OPL, showing no increase in the number (D) or length (E) of ectopic neurites (n = 52 sprouts from four control mice; n = 84 sprouts from four C3aR–/– mice; n = 67 sprouts from four CR3–/– mice). Scale bars = 25 μm. Data are represented as the mean ± SEM (D) or as beeswarm SuperPlots (E) in which individual horizontal cell sprout values are presented together with the mean from each animal ± the SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33177995), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50028_chicken-polyclonal-calbindin-d-28k-antibody-31020241613643.jpg)
![Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Myh10 & Myh14 are the NM2 isoforms expressed in the distal & connecting renal tubules. Adult kidney sections were stained for calbindin‐D28K, Aqp2 & one of the NM2 isoforms. Myh9 (A–D) was not expressed in tubules positive for calbindin‐D28K & Aqp2; although adjacent tubules were positive for Myh9 (arrow). Myh10 (E–H) was expressed at low levels at the cell–cell junctions in calbindin‐D28K only positive tubules (white arrowhead), & in tubules positive for both calbindin‐D28K & Aqp2, Myh10 localized to cell–cell junctions, apical, & basolateral membrane (F & H, yellow arrowhead). Myh14 (I–L) was expressed on the apical membrane of calbindin‐D28K & Aqp2 null cells (arrowheads) in the distal tubular segments. Sections were mounted with vectashield containing DAPI to stain the nucleus (blue; D, H, & L). Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29208685), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50028_chicken-polyclonal-calbindin-d-28k-antibody-310202416114730.jpg)
![Western Blot: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of cow cerebellum lysate were probed with NBP2-50048. NBP2-50048 binds strongly and specifically to Calbindin at 28 kDa.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-4H7-Western-Blot-NBP2-50048-img0002.jpg "Western Blot: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of cow cerebellum lysate were probed with NBP2-50048. NBP2-50048 binds strongly and specifically to Calbindin at 28 kDa.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Rat brain cerebellum (Middle) and cortex (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were co-stained with NBP2-50048 (red) and anti-MeCP2 rabbit antibody (NBP2-50060, green). Calbindin is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum, and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex. MeCP2 is universally expressed in the nuclei of almost all neurons. As a result, calbindin-expressing cell is strongly labeled with red in soma, but the nucleus appears to be yellow. Blue is Dapi nucleus staining. Insets are high magnification images of the boxed areas.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-50048-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of rat brain cerebellum section stained with mouse mAb to calbindin, NBP2-50048, dilution 1:1,000, in green, and costained with rabbit pAb to calretinin, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. Calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum. In contrast the calretinin antibody stains granule cells, in the granualar layer, and their processes in the molecular layer.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-50048-img0004.jpg)
![Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249425488.jpg "Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249362752.jpg)
![Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249432860.jpg)
![Immunohistochemistry-Paraffin: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL1787] (NBP3-25390) diluted at 1:250. Red: NF-H, a neural marker, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-212024939116.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin antibody [HL1787] detects Calbindin protein at cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin stained by Calbindin antibody [HL1787] (NBP3-25390) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249342761.jpg)
![Immunohistochemistry-Frozen: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin + Calretinin antibody [HL1787] detects Calbindin + Calretinin protein at cytoplasm by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: Calbindin + Calretinin stained by Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249432881.jpg)
![Immunohistochemistry-Paraffin: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL1787] (NBP3-25390) diluted at 1:250. Red: NF-H, a neural marker, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-2120249342739.jpg)
![Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25390_rabbit-calbindin-1-2-mab-hl1787-azide-and-bsa-free-212024940898.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining in human kidney and lymph node tissues using anti-CALB1 antibody. Corresponding CALB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP1-86664-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining in human kidney and lymph node tissues using anti-CALB1 antibody. Corresponding CALB1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cerebellum, kidney, liver and lymph node using Anti-CALB1 antibody NBP1-86664 (A) shows similar protein distribution across tissues to independent antibody NBP2-38798 (B).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP1-86664-img0013.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP1-86664] - Analysis in human kidney tissue.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP1-86664-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86664-img0012.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human lymph node using Anti-CALB1 antibody NBP1-86664.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP1-86664-img0019.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP1-86664-img0009.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human liver.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP1-86664-img0014.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cerebellum.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP1-86664-img0015.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human lymp node.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP1-86664-img0017.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human kidney using Anti-CALB1 antibody NBP1-86664.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP1-86664-img0018.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024941471.jpg "Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415939.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-212024942549.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249393563.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249415961.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249371590.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2379) - Azide and BSA Free [NBP3-25392] - Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2379] (NBP3-25392) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25392_rabbit-calbindin-d-28k-mab-hl2379-azide-and-bsa-free-2120249355523.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249415924.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-212024935136.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249432872.jpg)
![Immunohistochemistry-Frozen: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:300. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249393591.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249415945.jpg)
![Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249342788.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25391_rabbit-calbindin-d-28k-mab-hl2378-azide-and-bsa-free-2120249374965.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining in human kidney and lymph node tissues using anti-CALB1 antibody. Corresponding CALB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining in human kidney and lymph node tissues using anti-CALB1 antibody. Corresponding CALB1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human cerebellum, kidney, liver and lymph node using Anti-CALB1 antibody NBP2-38798 (A) shows similar protein distribution across tissues to independent antibody NBP1-86664 (B).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0005.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-38798] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-38798-img0002.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human lymph node using Anti-CALB1 antibody NBP2-38798.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0009.jpg)
![Immunohistochemistry: Calbindin D-28K Antibody [NBP2-38798] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-NBP2-38798-img0001.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0003.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human cerebellum.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0006.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human liver.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0007.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-38798] - Staining of human kidney using Anti-CALB1 antibody NBP2-38798.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-38798-img0008.jpg)
![Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Western-Blot-NBP2-50050-img0004.jpg "Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Adult mouse cortex (Left) and cerebellum (Right) sections were stained with NBP2-50050 in red, and co-stained with rabbit polyclonal anti-RBFOX3/NeuN antibody (NBP1-92716, green). Calbindin is expressed in a subset of interneurons in the cortex (Left) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (Right). RBFOX3/NeuN expresses in most neurons; as a result, cells positive for Calbindin appear to be yellow. Insets are high magnification images of the boxed area. Blue is Dapi nucleus staining that labels DNA.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunocytochemistry-Immunofluorescence-NBP2-50050-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of rat brain cerebellum section stained with mouse mAb to Calbindin D-28K, dilution 1:2,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The Calbindin D-28K antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunohistochemistry-Free-Floating-NBP2-50050-img0006.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-99063-img0003.jpg "Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063] - Immunofluorescence staining of Calbindin D-28K in 293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Calbindin D-28K polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99063-img0001.jpg)
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063] - Immunochemical staining of human Calbindin D-28K in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-99063-img0004.jpg)
![Immunoprecipitation: Calbindin D-28K Antibody [NBP2-99063] - Calbindin D-28K was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 4 ul anti-Calbindin D-28K rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-Calbindin D-28K rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunoprecipitation-NBP2-99063-img0002.jpg)