| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-78486SS | |||
| Azide and BSA Free | NBP2-80884 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1057-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78292SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5267-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73042 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3647-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95105-0.02ml | |||
![Knockout Validated: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Knockout-Validated-NB100-78486-img0011.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kDa full-length NOTCH1 and 110-120 kDa cleaved NOTCH 1 (NICD).](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Western-Blot-NB100-78486-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Immunocytochemistry-Immunofluorescence-NB100-78486-img0008.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) [NB100-78486] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in epithelial cells was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Immunohistochemistry-Paraffin-NB100-78486-img0010.jpg)
![Flow Cytometry: Notch-1 Antibody (mN1A) [NB100-78486] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Flow-Cytometry-NB100-78486-img0007.jpg)
![Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP1-78292-img0005.jpg "Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB image submitted by a verified customer review.")
![Immunohistochemistry: Notch-1 Antibody [NBP1-78292] - Staining of Notch1 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunohistochemistry-NBP1-78292-img0003.jpg)
![Western Blot: Notch-1 Antibody [NBP1-78292] - Analysis of Cleaved Notch1 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP1-78292-img0002.jpg)
![Simple Western: Notch-1 Antibody [NBP1-78292] - Lane view shows a specific band for Notch-1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Simple-Western-NBP1-78292-img0004.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17beta-estradiol. Lane 3: MCF-7 + 10 uM gamma secretase inhibitor. Load: 35 ug per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for Notch1.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0010.jpg "Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17beta-estradiol. Lane 3: MCF-7 + 10 uM gamma secretase inhibitor. Load: 35 ug per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for Notch1.")
![Immunohistochemistry: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Analysis of Tissue: Exocrine glands of human pancreas Fixation: FFPE Primary antibody: Notch1 antibody at 1:200 Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Immunohistochemistry-NB300-251-img0007.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Notch 1 Antibody [NB 300-251]- at 1:500, against myc-tagged transiently transfected mouse Notch constructs in 293 cells.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0003.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0009.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0011.jpg)
![Western Blot: Notch-1 Antibody (3E12) [NBP1-48289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Western-Blot-NBP1-48289-img0030.jpg "Western Blot: Notch-1 Antibody (3E12) [NBP1-48289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.")
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HepG2 cells using anti Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunocytochemistry-Immunofluorescence-NBP1-48289-img0032.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunocytochemistry-Immunofluorescence-NBP1-48289-img0016.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0017.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0018.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0019.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0020.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0021.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0022.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0023.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0024.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0025.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0026.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0027.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0028.jpg)
![Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP2-95105-img0006.jpg "Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.")
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody [NBP2-95105] - Immunofluorescence analysis of U2OS cells using Notch-1 Rabbit pAb (NBP2-95105) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95105-img0011.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody [NBP2-95105] - Paraffin-embedded rat testis using Notch-1 .](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95105-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody [NBP2-95105] - Immunofluorescence analysis of A-549 cells using Notch-1 Rabbit pAb (NBP2-95105) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95105-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody [NBP2-95105] - Immunofluorescence analysis of HeLa cells using Notch-1 Rabbit pAb (NBP2-95105) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95105-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody [NBP2-95105] - Immunofluorescence analysis of NIH/3T3 cells using Notch-1 Rabbit pAb (NBP2-95105) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95105-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody [NBP2-95105] - Immunofluorescence analysis of PC-12 cells using Notch-1 Rabbit pAb (NBP2-95105) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95105-img0010.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody [NBP2-95105] - Paraffin-embedded human placenta using Notch-1 .](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95105-img0003.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody [NBP2-95105] - Paraffin-embedded mouse testis using Notch-1 .](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95105-img0004.jpg)
![Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP2-24771-img0002.jpg "Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.")