![Western Blot: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kDa full-length NOTCH1 and 110-120 kDa cleaved NOTCH 1 (NICD). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Azide-and-BSA-Free-Western-Blot-NBP2-80884-img0001.jpg "Western Blot: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kDa full-length NOTCH1 and 110-120 kDa cleaved NOTCH 1 (NICD). Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready, KO |
| Clone | mN1A |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Mouse Notch1 protein [Uniprot: Q01705] |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NOTCH1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The mN1A monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms. This antibody is CyTOF ready. |
| Theoretical MW | 272.505 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Notch-1 Antibody (NBP2-80884)Find related products by research area.
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Notch1 - A multifunctional transmembrane receptor Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process... Read full blog post. |
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| Gene Symbol | NOTCH1 |
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80884-img0002.jpg "Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue). Image from the standard format of this antibody.")
![Immunohistochemistry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous p](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80884-img0003.jpg "Immunohistochemistry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous p")
![Flow Cytometry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80884-img0004.jpg "Flow Cytometry: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.")
![Knockout Validated: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Azide-and-BSA-Free-Knockout-Validated-NBP2-80884-img0005.jpg "Knockout Validated: Notch-1 Antibody (mN1A) - Azide and BSA Free [NBP2-80884] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")