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Cystatin E/M/CST6 Antibody [Unconjugated]

 (6 Publications)
 (1 Review)
 
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Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cystatin E/M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1286) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin E/M at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cystatin E/M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1286) followed by HRP-conjugated ...read more
Simple Western lane view shows lysates of human skin tissue, loaded at 0.2 mg/mL. A specific band was detected for Cystatin E/M at approximately 13 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Cystatin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1286
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Catalog# & Formulation Size Price
Carrier Free
AF1286-SP
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Cystatin E/M/CST6 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin E/M
Arg29-Met149
Accession # Q15828
Specificity
Detects human Cystatin E/M in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Cystatin E/M is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CST6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1286 in the following applications:
Publications
Read Publications using
AF1286 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin E/M/CST6 Antibody [Unconjugated]

  • CST6
  • cystatin 6
  • Cystatin E/M
  • cystatin M
  • cystatin M/E
  • Cystatin-6
  • cystatin-E
  • cystatin-M
  • cysteine proteinase inhibitor

Background

Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily (1, 2). It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning (1, 2). However, its protein was found only in skin and sweat glands by a third group (3). In addition to being a cysteine protease inhibitor, cystatin E/M is also a substrate for transglutaminases (3). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age (4). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (5).

  1. Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
  2. Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
  3. Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.
  4. Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
  5. Hong, J. et al. (2002) J. Neurochem. 81:922.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cystatin E/M/CST6 Antibody (AF1286)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC, IHC, Neutralization, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
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(1)
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(3)
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(4)
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Showing Publications 1 - 6 of 6.
Publications using AF1286 Applications Species
NN Lunde, MH Haugen, KB Bodin Lars, I Damgaard, SJ Pettersen, R Kasem, W Rut, M Drag, M Poreba, HT Johansen, R Solberg Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions Biochimie, 2017-05-17;0(0):. 2017-05-17 [PMID: 28528272] (Western Blot, Human) Western Blot Human
Mads H. Haugen, Harald T. Johansen, Solveig J. Pettersen, Rigmor Solberg, Klaudia Brix, Kjersti Flatmark et al. Correction: Nuclear Legumain Activity in Colorectal Cancer PLoS ONE 2013-09-19 [PMID: 24086214]
Mads H. Haugen, Harald T. Johansen, Solveig J. Pettersen, Rigmor Solberg, Klaudia Brix, Kjersti Flatmark et al. Nuclear Legumain Activity in Colorectal Cancer PLoS ONE 2013-01-10 [PMID: 23326369]
Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis. Cell Res., 2012-06-12;22(9):1356-73. 2012-06-12 [PMID: 22688893] (IHC, Neutralization, Western Blot, Human) IHC, Neutralization, Western Blot Human
Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad O, Maelandsmo GM, Solberg R Cystatin E/M suppresses legumain activity and invasion of human melanoma. BMC Cancer, 2010-01-15;10(1):17. 2010-01-15 [PMID: 20074384] (Western Blot, Human) Western Blot Human
Qiu J, Ai L, Ramachandran C, Yao B, Gopalakrishnan S, Fields CR, Delmas AL, Dyer LM, Melnick SJ, Yachnis AT, Schwartz PH, Fine HA, Brown KD, Robertson KD Invasion suppressor cystatin E/M (CST6): high-level cell type-specific expression in normal brain and epigenetic silencing in gliomas. Lab. Invest., 2008-07-07;88(9):910-25. 2008-07-07 [PMID: 18607344] (ICC, Human) ICC Human

Review for Cystatin E/M/CST6 Antibody (AF1286) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1286:
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(1)
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  5
reviewed by:
Verified Customer
 WB Human 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22902879
SpeciesHuman

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Application: WB
Species: Human
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Bioinformatics

Gene Symbol CST6
Uniprot