Antibody database

Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and GPR49/LGR5 antibodies.

We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.

A large percentage of the products in our antibody catalog are used for cancer research. Some oncogenes are expressed in several types of tumor, while others are quite specific. For example, there are several products on our antibody database which target genes specific to nasopharyngeal carcinomas (NPC), such as UBAP1 and MMP9. Recently, a Chinese cancer study, which used no fewer than 10,000 volunteer subjects, uncovered 3 new susceptibility genes for NPC.

The G-protein coupled receptor (GPCR) family constitutes one of the largest protein families in the mammalian kingdom, with over 800 proteins so far discovered. Our GPCR antibody catalog covers all 6 classes, with antibodies raised against Metabotropic Glutamate/Pheromone receptors; Rhodopsin-like receptors; Secretin receptors; Fungal Mating Pheromone receptors; cAMP receptors and Frizzled/Smoothened receptors. The majority are of the Class A (Rhodopsin-like) class.

We at Novus Biologicals have a broad antibody database covering the area of autophagy - over 1400 reagents in total. Autophagy is the bulk degradation of cytoplasmic components - literally, self-digestion of the cell. Double-membrane vesicles, called autophagosomes, carry unwanted cell components to the lysosomes within an inner autophagic membrane. They then fuse, liberating the autophagic body and its contents into the lumen of the vacuole for degradation. This is a complex process involving at least 16 proteins.

We at Novus Biologicals have a large antibody database devoted to signalling pathways. These underpin every area of molecular biological research, including cancer. Among our cell signalling antibodies is one targeted to DLL4 (Delta-like protein 4). DLL4 is a homologue of the Drosophila delta gene, known to encode DSL-domain ligands on the Notch signaling pathway.

Most commercially available antibodies produced with traditional immunization technology, using peptides or other immunogens isolated and purified from cell lysates.

Typically, mice are immunized with the antigen in groups, varying the mouse strain and antigen dosage. Pre-immune and immune sera are evaluated and mice showing the best immune response are selected for somatic cell fusion. This is the fusion of antibody-producing lymphocytes with proprietary myeloma cells from a non-antibody producing source, creating a hybridoma.

Novus Biologicals recently added novel GAT (Genomic Antibody Technology) reagents to our antibody catalog, following the formation of a partnership with SDIX, creators of the technique. SDIX's GAT antibodies represent a totally new way to think about protein immunogens.

Novus Biologicals offers an extensive antibody databasecovering all areas of biological research. Recently, we dramatically increased the scope of our antibody catalog by adding over 800 antibodies created  using Genomic Antibody Technology (GAT), through a new partnership with SDIX (Strategic Diagnostics, Inc.). As we begin creating novel GAT reagents of our own, this database will expand still further – as there are many GAT antibodiesstill to be created.

Ago2, also called eIF2C2, antibody is one of 37 reagents targeted to the Argonaute protein family that we at Novus Biologicals have in our antibody catalogue. Argonaute proteins are encoded by genes which play an important role in regulating the control of gene expression by miRNA (microRNA). Recently, a new antibody study showed that the role of Ago2 may be to help generate production of miRNA, rather than simply moderate its function.