Immunogen | Alpha-Smooth Muscle Actin Antibody is made to a synthetic peptide corresponding to N-terminus of human alpha smooth muscle isoform of actin. |
Localization | Cytoplasmic |
Marker | Mesenchymal Cell Marker |
Specificity | This alpha-sma antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | ACTA2 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Use in Flow reported in scientific literature (PMID:34571434). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25783396). ; Western Blot (PMID 27029074). ; ICC/IF (PMID 26417067). and IHC free-floating sections (25919028). . |
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Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Publications |
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Storage | Store at 4C. Do not freeze. |
Buffer | PBS (pH 7.6) and 1.0% BSA |
Preservative | 0.1% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for alpha-Smooth Muscle Actin Antibody (NB600-531)Find related products by research area.
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Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t... Read full blog post. |
Understanding Actin Alpha 2 Smooth Muscle Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated... Read full blog post. |
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