EGLN2/PHD1 Antibody

HIF prolyl 4-hydroxylases (PHD) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that facilitate physiological reactions to hypoxic stress by modulating HIF1alpha expression levels. HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). HIF Prolyl Hydroxylase 1 (HIF-PH1/EGLN2/PHD1) is localized in the nucleus and interacts with SIAH2, LIMD1, WTIP and AJUBA. In normal oxygen conditions, HIF-PH1 catalyzes the post-translational formation of 4-hydroxyproline in HIF- alpha and hydroxylates a specific prolines found in NODD/CODD of HIF1A, and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. VHL (von Hippel-Lindau ubiquitination) complex then targets hydroxylated HIFs to facilitate their proteasomal degradation. Under hypoxic stress conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH1 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle, tumor angiogenesis and susceptibility to normoxic oxidative neuronal death.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: EM, ICC/IF, IF/IHC, IHC-P, IP, WB.

Showing Publications 1 - 10 of 47. Show All 47 Publications. Collapse Publications.

Publications using NB100-310	Applications	Species
Melling N, Reeh M, Ghadban T et al. RAI3 expression is not associated with clinical outcomes of patients with non-small cell lung cancer Research Square 2023-01-23 [PMID: 36781501]
Meng X, Lin Z, Cao S et al. Estrogen-mediated downregulation of HIF-1 alpha signaling in B lymphocytes influences postmenopausal bone loss Bone research 2022-02-17 [PMID: 35177582] (WB)	WB
van Kuijk K, Demandt JAF, Perales-PatOn J et al. DEFICIENCY OF MYELOID PHD PROTEINS AGGRAVATES ATHEROGENESIS VIA MACROPHAGE APOPTOSIS AND PARACRINE FIBROTIC SIGNALING: Atherogenic effects of myeloid PHD knockdown Cardiovascular research 2021-04-26 [PMID: 33913468] (IF/IHC, Human)	IF/IHC	Human
Shi M, Dai WQ, Jia RR et al. APCCDC20-mediated degradation of PHD3 stabilizes HIF-1a and promotes tumorigenesis in hepatocellular carcinoma Cancer Lett 2020-10-09 [PMID: 33039559] (WB, IP, Mouse)	WB, IP	Mouse
Zurlo G, Liu X, Takada M et al. Prolyl hydroxylase substrate adenylosuccinate lyase is an oncogenic driver in triple negative breast cancer Nat Commun. 2019-11-15 [PMID: 31729379] (WB, Human)	WB	Human
El Gammal AT, Melling N, Reeh M et al. High levels of RAI3 expression is linked to shortened survival in esophageal cancer patients Exp. Mol. Pathol. 2019-01-29 [PMID: 30707896] (IHC-P, Human)	IHC-P	Human
Henze At, Garvalov Bk, Seidel S et al. Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR. Nat Commun. 2014-11-25 [PMID: 25420773]
Yogev O, Lagos D, Enver T, Boshoff C. Kaposi's Sarcoma Herpesvirus MicroRNAs Induce Metabolic Transformation of Infected Cells. PLoS Pathog. 2014-09-01 [PMID: 25255370] (WB, Human) Details: EGLN2/PHD1 antibody used for WB in experiments involving human primary dermal microvascular lymphatic endothelial cells (LEC).	WB	Human
Botting KJ, McMillen IC, Forbes H, Nyengaard, JR. Chronic hypoxemia in late gestation decreases cardiomyocyte number but does not change expression of hypoxia-responsive genes. J Am Heart Assoc. 2014-08-02 [PMID: 25085511] (WB)	WB
Zheng Xingnan, Zhai Bo, Koivunen Peppi et al. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev. 2014-07-01 [PMID: 24990963] (WB, Mouse)	WB	Mouse
Peurala E, Koivunen P, Bloigu R, Haapasaari KM, Jukkola-Vuorinen A. Expressions of individual PHDs associate with good prognostic factors and increased proliferation in breast cancer patients. Breast Cancer Res Treat;133(1):179-88. 2012-05-01 [PMID: 21877141] (IF/IHC, Human)	IF/IHC	Human
Pyott SM, Schwarze U, Christiansen HE et al. Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes. Hum Mol Genet. 2011-02-13 [PMID: 21282188]
Henze AT, Riedel J, Diem T et al. Prolyl hydroxylases 2 and 3 act in gliomas as protective negative feedback regulators of hypoxia-inducible factors. Cancer Res;70(1):357-66. 2010-01-01 [PMID: 20028863]
Schodel J, Klanke B, Weidemann A et al. HIF-prolyl hydroxylases in the rat kidney: physiologic expression patterns and regulation in acute kidney injury. Am J Pathol 2009-05-01 [PMID: 19349364] (WB, Mouse)	WB	Mouse
Schultz K, Murthy V, Tatro JB et al. Prolyl hydroxylase 2 deficiency limits proliferation of vascular smooth muscle cells by hypoxia-inducible factor-1{alpha}-dependent mechanisms. Am J Physiol Lung Cell Mol Physiol;296(6):L921-927. 2009-01-01 [PMID: 19304911]
Lee K, Lynd JD, O'Reilly S et al. The biphasic role of the hypoxia-inducible factor prolyl-4-hydroxylase, PHD2, in modulating tumor-forming potential. Mol Cancer Res;6(5):829-42. 2008-05-01 [PMID: 18505927]
Mikhaylova, O, Ignacak, M et al. The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress. Mol Cell Biol;28(8):2701-17. 2008-04-01 [PMID: 18285459]
Takeda K, Aguila HL, Parikh NS et al. Regulation of adult erythropoiesis by prolyl hydroxylase domain proteins. Blood;111(6):3229-35. 2008-03-15 [PMID: 18056838]
Groenman F, Rutter M, Caniggia I et al. Hypoxia-inducible factors in the first trimester human lung. J Histochem Cytochem 2007-04-01 [PMID: 17189520]
Li N et al. Expression actions of HIF prolyl-4-hydroxylase in the rat kidneys. Am J Physiol Renal Physiol 292: F207 - F216. 2007-01-01 [PMID: 16885149]
McMahon, S et al. PROTEIN SYNTHESIS, POST-TRANSLATION MODIFICATION, DEGRADATION: TGF beta 1 induces HIF-1 stabilization through selective inhition of PHD2 expression. J Biol Chem, 10.1074/jbc.M604507200. 2006-01-01 [PMID: 16815840]
Ietta, F et al. Dynamic HIF-1alpha regulation during human placental development. Biol Reprod. 2006-01-01 [PMID: 16611863]
Asikainen, TM et al. Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells explants of primate lung. PNAS 102(29): 10212-10217. 2005-01-01 [PMID: 16009933] (WB, Human)	WB	Human
Haag D. Rolle der Siah-Ubiquitinligasen in der Entstehung der pulmonalen Hypertonie und daraus resultierenden rechtsventrikularen Hypertrophie. Einleitung. 2014-01-01 (WB, Mouse)	WB	Mouse
Kozlova N, Mennerich D, Samoylenko A et al. The pro-oncogenic adaptor CIN85 inhibits hypoxia-inducible factor prolyl hydroxylase-2. Cancer Res 2019-05-31 [PMID: 31142511]
Zhang L, Peng S, Dai X et al. Tumor suppressor SPOP ubiquitinates and degrades EglN2 to compromise growth of prostate cancer cells Cancer Lett 2017-01-13 [PMID: 28089830]
Takada M, Zhuang M, Inuzuka H et al. EglN2 contributes to triple negative breast tumorigenesis by functioning as a substrate for the FBW7 tumor suppressor. Oncotarget. 2017-01-24 [PMID: 28036276] (WB, Human)	WB	Human
Zhang J, Wang C, Chen X et al. EglN2 associates with the NRF1-PGC1alpha complex and controls mitochondrial function in breast cancer. EMBO J 2015-12-02 [PMID: 26492917] (WB, Human)	WB	Human
Deschoemaeker S, Di Conza G, Lilla S et al. PHD1 regulates p53-mediated colorectal cancer chemoresistance. EMBO Mol Med. 2015-08-19 [PMID: 26290450] (WB, Human)	WB	Human
Hogel H, Miikkulainen P, Bino L, Jaakkola PM. Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27. Mol. Cancer. 2015-07-30 [PMID: 26223520] (WB, Human) Details: PHD1 antibody used for WB on 786-O cell under hypoxia (1% O2) and normoxia (21 % O2) by siPHD1 (Figure 1C).	WB	Human
Chu HX, Jones NM. Changes in Hypoxia-Inducible Factor-1 (HIF-1) and Regulatory Prolyl Hydroxylase (PHD) Enzymes Following Hypoxic-Ischemic Injury in the Neonatal Rat Neurochem. Res. 2015-06-25 [PMID: 26108712] (WB, Rat)	WB	Rat
Kim J, Kwak HJ, Cha JY et al. The role of prolyl hydroxylase domain protein (PHD) during rosiglitazone-induced adipocyte differentiation. J Biol Chem 2013-12-12 [PMID: 24338020] (WB, Mouse)	WB	Mouse
Rawluszko AA, Bujnicka KE, Horbacka K et al. Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer. BMC Cancer. 2013-11-06 [PMID: 24195777] (WB, Human)	WB	Human
Scholz CC, Cavadas MA, Tambuwala MM et al. Regulation of IL-1beta-induced NF-kB by hydroxylases links key hypoxic and inflammatory signaling pathways. Proc Natl Acad Sci U S A. 2013-11-12 [PMID: 24145445] (WB, Human)	WB	Human
Tan JT, Prosser HC, Vanags LZ et al. High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1alpha. FASEB J. 2013-09-10 [PMID: 24022405] (WB, Human)	WB	Human
Mazzon M, Peters NE, Loenarz C et al. A mechanism for induction of a hypoxic response by vaccinia virus. Proc Natl Acad Sci U S A 2013-07-23 [PMID: 23836663] (WB, Human)	WB	Human
Palomaki S, Pietila M, Laitinen S et al. HIF-1alpha is upregulated in human mesenchymal stem cells. Stem Cells 2013-09-01 [PMID: 23744828]
Duran RV, Mackenzie ED, Boulahbel H et al. HIF-independent role of prolyl hydroxylases in the cellular response to amino acids Oncogene 2012-10-22 [PMID: 23085753] (WB, Human)	WB	Human
Kunze R, Zhou W, Veltkamp R et al. Neuron-specific prolyl-4-hydroxylase domain 2 knockout reduces brain injury after transient cerebral ischemia. Stroke 2012-10-01 [PMID: 22933585] (Mouse)		Mouse
Xie G, Zheng L, Ou J et al. Low expression of prolyl hydroxylase 2 is associated with tumor grade and poor prognosis in patients with colorectal cancer Exp Biol Med 2012-07-01 [PMID: 22802519] (IF/IHC, Human)	IF/IHC	Human
Yan B, Jiao S, Zhang HS et al. Prolyl hydroxylase domain protein 3 targets Pax2 for destruction. Biochem Biophys Res Commun. 2011-06-01 [PMID: 21575608]
Wiley M, Sweeney KR, Chan DA et al. Toxoplasma gondii activates hypoxia-inducible factor (HIF) by stabilizing the HIF-1alpha subunit via type I activin-like receptor kinase receptor signaling. J Biol Chem. 2010-08-27 [PMID: 20581113] (WB, Human)	WB	Human
Hyvarinen J, Hassinen IE, Sormunen R et al. Hearts of hypoxia-inducible factor prolyl 4-hydroxylase-2 hypomorphic mice show protection against acute ischemia-reperfusion injury. J Biol Chem. 2010-04-30 [PMID: 20185832] (WB, Mouse)	WB	Mouse
Chan DA, Kawahara TL, Sutphin PD et al. Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment. Cancer Cell 2009-06-02 [PMID: 19477431] (WB)	WB
Khan Z, Michalopoulos GK, Stolz DB. Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation. Am J Pathol 2006-10-01 [PMID: 17003483] (WB, EM, IHC-P, ICC/IF, Rat)	WB, EM, IHC-P, ICC/IF	Rat
Kariuki TM, Van Dam GJ, Deelder AM et al. Previous or ongoing schistosome infections do not compromise the efficacy of the attenuated cercaria vaccine. Infect Immun 2006-07-01 [PMID: 16790771]
Baek JH, Mahon PC, Oh J et al. OS-9 interacts with hypoxia-inducible factor 1alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1alpha. Mol Cell;17(4):503-12. 2005-02-18 [PMID: 15721254] (IP, Human)	IP	Human
Show All 47 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Helder Andre	IF	Human	05/14/2015	View Summary Application Immunofluorescence Sample Tested ARPE-19 cells Species Human
Enlarge	4 reviewed by: Verified Customer	WB	Human	04/30/2014	View Summary Application Western Blot Sample Tested Hela whole cell lysate Species Human
	4 reviewed by: Verified Customer	WB	Human	10/01/2013	View Summary Application Western Blot Species Human

Array NB100-310

• EGLN2/PHD1 Antibodies
• EGLN2/PHD1 Lysates
• EGLN2/PHD1 Proteins