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![Western Blot: EGLN2/PHD1 Antibody [NBP3-05472] - Total protein from human HeLa, K562, HepG2 and U87 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-EGLN2/PHD1 (NBP2-59179) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-Western-Blot-NBP3-05472-img0001.jpg "Western Blot: EGLN2/PHD1 Antibody [NBP3-05472] - Total protein from human HeLa, K562, HepG2 and U87 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-EGLN2/PHD1 (NBP2-59179) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: EGLN2/PHD1 Antibody [NBP3-05472] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-EGLN2/PHD1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05472-img0002.jpg)
![Western Blot: EGLN2/PHD1 Antibody [NBP2-92974] - Analysis of extracts of various cell lines, using EGLN2/PHD1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-Western-Blot-NBP2-92974-img0002.jpg "Western Blot: EGLN2/PHD1 Antibody [NBP2-92974] - Analysis of extracts of various cell lines, using EGLN2/PHD1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunoprecipitation: EGLN2/PHD1 Antibody [NBP2-92974] - Analysis of 150ug extracts of HeLa cells using EGLN2/PHD1 at a dilition of 1:1000.](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-Immunoprecipitation-NBP2-92974-img0001.jpg)
![Western Blot: EGLN2/PHD1 Antibody (2G9M7) [NBP3-16265] - Analysis of extracts of various cell lines, using EGLN2/PHD1 Rabbit mAb (NBP3-16265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-2G9M7-Western-Blot-NBP3-16265-img0001.jpg "Western Blot: EGLN2/PHD1 Antibody (2G9M7) [NBP3-16265] - Analysis of extracts of various cell lines, using EGLN2/PHD1 Rabbit mAb (NBP3-16265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: EGLN2/PHD1 Antibody (S01-7G1) [NBP3-15114] - Western blot detection of EGLN2/PHD1 in Hela, A549, HL-60, U2OS, U87-MG cell lysates using EGLN2/PHD1 Rabbit mAb (1:1000 diluted). Observed band size: 44kDa.](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-S01-7G1-Western-Blot-NBP3-15114-img0001.jpg "Western Blot: EGLN2/PHD1 Antibody (S01-7G1) [NBP3-15114] - Western blot detection of EGLN2/PHD1 in Hela, A549, HL-60, U2OS, U87-MG cell lysates using EGLN2/PHD1 Rabbit mAb (1:1000 diluted). Observed band size: 44kDa.")
![SDS-Page: Recombinant Human EGLN2/PHD1 Protein [H00112398-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-EGLN2-PHD1-Protein-SDS-Page-H00112398-P01-img0002.jpg "SDS-Page: Recombinant Human EGLN2/PHD1 Protein [H00112398-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: EGLN2/PHD1 Antibody (2G3) [H00112398-M01] - Western Blot detection against Immunogen (70.51 KDa) .](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-2G3-Western-Blot-H00112398-M01-img0001.jpg "Western Blot: EGLN2/PHD1 Antibody (2G3) [H00112398-M01] - Western Blot detection against Immunogen (70.51 KDa) .")
![Sandwich ELISA: EGLN2/PHD1 Antibody (2G3) [H00112398-M01] - Detection limit for recombinant GST tagged EGLN2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-2G3-Sandwich-ELISA-H00112398-M01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN2/PHD1 Antibody [NBP2-76551] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EGLN2-PHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76551-img0001.jpg "Immunocytochemistry/Immunofluorescence: EGLN2/PHD1 Antibody [NBP2-76551] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Western Blot: PHD1/HIF Prolyl Hydroxylase 1 Overexpression Lysate (Adult Normal) [NBL1-10155] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF Prolyl Hydroxylase 1.](http://images.novusbio.com/fullsize/PHD1-HIF-Prolyl-Hydroxylase-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10155-img0002.jpg "Western Blot: PHD1/HIF Prolyl Hydroxylase 1 Overexpression Lysate (Adult Normal) [NBL1-10155] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF Prolyl Hydroxylase 1.")
![Western Blot: PHD1/HIF Prolyl Hydroxylase 1 Overexpression Lysate (Adult Normal) [NBL1-10154] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF Prolyl Hydroxylase 1.](http://images.novusbio.com/fullsize/PHD1-HIF-Prolyl-Hydroxylase-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10154-img0002.jpg "Western Blot: PHD1/HIF Prolyl Hydroxylase 1 Overexpression Lysate (Adult Normal) [NBL1-10154] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF Prolyl Hydroxylase 1.")