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FAT1 Antibody (FAT-1 3D7/1)

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Summary
Product Discontinued
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    • Catalog Number
      NB100-2693
    • Availability
      Product Discontinued

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FAT1 Antibody (FAT-1 3D7/1) Summary

Immunogen
Cytoplasmic domain of drosophila Fat
Localization
Cell Membrane
Predicted Species
Chimpanzee (92%). Backed by our 100% Guarantee.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
FAT1
Purity
Thiophilic Chromatography
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Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
3-8% gradient gels and wet transfer techniques are recommended for the visualisation of FAT1. Positive control(s): Wild type imaginal discs from third instar Drosophila larvae.
Reviewed Applications
Read 1 Review rated 4
using
NB100-2693 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Thiophilic Chromatography

Alternate Names for FAT1 Antibody (FAT-1 3D7/1)

  • FAT tumor suppressor 1
  • FAT tumor suppressor homolog 1 (Drosophila)

Background

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FAT1 Antibody (NB100-2693) (0)

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Review for FAT1 Antibody (NB100-2693) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Rat.

Reviews using NB100-2693:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot FAT1 NB100-2693
Enlarge 		4
reviewed by:
Verified Customer
 WB Human and Rat 03/03/2017
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Summary

ApplicationWestern Blot
Sample Testedmacrophage
SpeciesHuman and Rat
Lot596/05

Comments

Comments3-8 PERCENT TRIS ACETATE GEL
TRANSFER 10V OVERNIGHT
ANTIBODY 1:500
SHOWS HIGHER THAN 500 KDA BAND IN RAT, NOT IN HUMAN SAMPLES.

Product General Protocols

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Array NB100-2693

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Recent Reviews

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Western Blot FAT1 NB100-2693
Enlarge
 Verified Customer
03/03/2017
Application: WB
Species: Human and Rat
read this review

Bioinformatics

Gene Symbol FAT1
Entrez